Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13974 | 3' | -59.7 | NC_003521.1 | + | 208052 | 1.06 | 0.003206 |
Target: 5'- cUCUCGCCGUGAGGAAGCGGGAGGGAAg -3' miRNA: 3'- -AGAGCGGCACUCCUUCGCCCUCCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 19085 | 0.76 | 0.310136 |
Target: 5'- ---gGCCGUGAGGAGGagggggacgGGGAGGGAGa -3' miRNA: 3'- agagCGGCACUCCUUCg--------CCCUCCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 123355 | 0.76 | 0.316989 |
Target: 5'- -gUCGCCGggugugGAGGAGGCGGcGGGGGc- -3' miRNA: 3'- agAGCGGCa-----CUCCUUCGCC-CUCCCuu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 13891 | 0.75 | 0.352956 |
Target: 5'- aUUUCGCCG-GGGGGcccGGCGcGGAGGGGg -3' miRNA: 3'- -AGAGCGGCaCUCCU---UCGC-CCUCCCUu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 197275 | 0.74 | 0.383745 |
Target: 5'- --gCGCCGgcgGcGGGGAGCGGGAGGGc- -3' miRNA: 3'- agaGCGGCa--C-UCCUUCGCCCUCCCuu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 123523 | 0.72 | 0.495168 |
Target: 5'- aCUcCGUCGUGGGGcAGCGuGAGGGGAg -3' miRNA: 3'- aGA-GCGGCACUCCuUCGCcCUCCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 100718 | 0.71 | 0.570527 |
Target: 5'- --cCGCUGaugGAGGAGGCGGGGGGu-- -3' miRNA: 3'- agaGCGGCa--CUCCUUCGCCCUCCcuu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 151105 | 0.71 | 0.580179 |
Target: 5'- gUCggGCCGcUGGGGGauGGCGGG-GGGAAu -3' miRNA: 3'- -AGagCGGC-ACUCCU--UCGCCCuCCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 185721 | 0.69 | 0.671701 |
Target: 5'- gUCUCGCCGggugcgGAGGugaucgccGAGCGGGugcgccggagcgccuAGGGGc -3' miRNA: 3'- -AGAGCGGCa-----CUCC--------UUCGCCC---------------UCCCUu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 192420 | 0.69 | 0.677518 |
Target: 5'- cCUCGCCGagacucGAGGGAGacgaaGGaGAGGGGc -3' miRNA: 3'- aGAGCGGCa-----CUCCUUCg----CC-CUCCCUu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 239094 | 0.68 | 0.725359 |
Target: 5'- -gUCGCgG-GAGGAAcGCgagGGGAGGGGAc -3' miRNA: 3'- agAGCGgCaCUCCUU-CG---CCCUCCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 31032 | 0.68 | 0.725359 |
Target: 5'- gUUCGUgGUGguGGGggGUGGGAGGa-- -3' miRNA: 3'- aGAGCGgCAC--UCCuuCGCCCUCCcuu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 155379 | 0.68 | 0.73474 |
Target: 5'- uUCUCGCCGgcgcggacgacGAGGccGGCGGGGGGc-- -3' miRNA: 3'- -AGAGCGGCa----------CUCCu-UCGCCCUCCcuu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 128300 | 0.68 | 0.762363 |
Target: 5'- gCUCGCCGcggaaaucggUGuGGAAGCGGauaaAGGGGu -3' miRNA: 3'- aGAGCGGC----------ACuCCUUCGCCc---UCCCUu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 67258 | 0.67 | 0.771371 |
Target: 5'- -aUCGacCCGUGgcAGGAgGGCGGGcAGGGAGg -3' miRNA: 3'- agAGC--GGCAC--UCCU-UCGCCC-UCCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 232571 | 0.67 | 0.771371 |
Target: 5'- --cUGCUGUGuGGuGAGCGGGGGGcGAc -3' miRNA: 3'- agaGCGGCACuCC-UUCGCCCUCC-CUu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 105004 | 0.67 | 0.771371 |
Target: 5'- cUUCGCUGcUGcAGG-AGCGGGAacGGGAGc -3' miRNA: 3'- aGAGCGGC-AC-UCCuUCGCCCU--CCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 172911 | 0.67 | 0.780267 |
Target: 5'- cCUCGUCcuucuggGAGGAGGaGGGAGGGu- -3' miRNA: 3'- aGAGCGGca-----CUCCUUCgCCCUCCCuu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 7975 | 0.67 | 0.780267 |
Target: 5'- ---gGCCGgGAGGGuccgcGGCGGcGGGGGAGg -3' miRNA: 3'- agagCGGCaCUCCU-----UCGCC-CUCCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 28032 | 0.67 | 0.786422 |
Target: 5'- --aCGCCGcG-GGGAGCGGGAcccaacagaggcggGGGAGa -3' miRNA: 3'- agaGCGGCaCuCCUUCGCCCU--------------CCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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