Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14004 | 5' | -52.6 | NC_003521.1 | + | 113577 | 0.69 | 0.963364 |
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Target: 5'- --aCGCGg--UGGGGCUGGGCCucGGg -3' miRNA: 3'- cagGUGCauaGCCCUGACCUGGuuUC- -5' |
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| 14004 | 5' | -52.6 | NC_003521.1 | + | 69698 | 0.71 | 0.918757 |
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Target: 5'- gGUCCugGUGUCGGuGGCgGcGGCCcuGAGg -3' miRNA: 3'- -CAGGugCAUAGCC-CUGaC-CUGGu-UUC- -5' |
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| 14004 | 5' | -52.6 | NC_003521.1 | + | 7984 | 0.71 | 0.907051 |
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Target: 5'- gGUCCGCGgcggCGGGGgaGGuACCGGGGc -3' miRNA: 3'- -CAGGUGCaua-GCCCUgaCC-UGGUUUC- -5' |
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| 14004 | 5' | -52.6 | NC_003521.1 | + | 139076 | 0.73 | 0.858993 |
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Target: 5'- -cCCugGccUCGGGGCUGGACUAc-- -3' miRNA: 3'- caGGugCauAGCCCUGACCUGGUuuc -5' |
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| 14004 | 5' | -52.6 | NC_003521.1 | + | 81181 | 0.73 | 0.835282 |
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Target: 5'- gGUCCACGcaccaggccUCGGcGAuCUGGGCCAAAGc -3' miRNA: 3'- -CAGGUGCau-------AGCC-CU-GACCUGGUUUC- -5' |
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| 14004 | 5' | -52.6 | NC_003521.1 | + | 156103 | 0.74 | 0.792245 |
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Target: 5'- -aCUACGUGUaCGGGAucCUGGcACCGGAGg -3' miRNA: 3'- caGGUGCAUA-GCCCU--GACC-UGGUUUC- -5' |
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| 14004 | 5' | -52.6 | NC_003521.1 | + | 232433 | 0.75 | 0.764659 |
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Target: 5'- -cCUGCGUGUCGGaggucGACUGGACCAc-- -3' miRNA: 3'- caGGUGCAUAGCC-----CUGACCUGGUuuc -5' |
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| 14004 | 5' | -52.6 | NC_003521.1 | + | 189648 | 1.08 | 0.01096 |
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Target: 5'- cGUCCACGUAUCGGGACUGGACCAAAGc -3' miRNA: 3'- -CAGGUGCAUAGCCCUGACCUGGUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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