Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14111 | 3' | -51.9 | NC_003521.1 | + | 211418 | 0.66 | 0.997093 |
Target: 5'- -cCGggGCCcggacuguccucGUCGUcgUCCUCCUCg-- -3' miRNA: 3'- uaGCaaCGG------------CAGCAa-AGGAGGAGauc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 119051 | 0.66 | 0.997093 |
Target: 5'- cGUCGgggUGCUGgagCGc-UCCUCCUCUu- -3' miRNA: 3'- -UAGCa--ACGGCa--GCaaAGGAGGAGAuc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 212582 | 0.66 | 0.996586 |
Target: 5'- cUCGgcGCCGUC--UUCUUCCUCc-- -3' miRNA: 3'- uAGCaaCGGCAGcaAAGGAGGAGauc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 183066 | 0.66 | 0.995355 |
Target: 5'- gAUCGUcGCCGUCGg-UCCUgUaCUGGg -3' miRNA: 3'- -UAGCAaCGGCAGCaaAGGAgGaGAUC- -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 47585 | 0.66 | 0.995355 |
Target: 5'- -aCGgugGCgGUCGgagUCCUCCUCc-- -3' miRNA: 3'- uaGCaa-CGgCAGCaa-AGGAGGAGauc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 71137 | 0.66 | 0.995355 |
Target: 5'- cUCGUcGCCGcC---UCCUCCUCUGc -3' miRNA: 3'- uAGCAaCGGCaGcaaAGGAGGAGAUc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 93330 | 0.67 | 0.990655 |
Target: 5'- uUCGggGCCGUg--UUCCUCaUCUGGg -3' miRNA: 3'- uAGCaaCGGCAgcaAAGGAGgAGAUC- -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 95853 | 0.68 | 0.98796 |
Target: 5'- cGUCGU--CCGUCcucUCCUCCUCUGu -3' miRNA: 3'- -UAGCAacGGCAGcaaAGGAGGAGAUc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 25013 | 0.68 | 0.986405 |
Target: 5'- uAUCGcUGCCGUCcuUUUCCUCCggCUc- -3' miRNA: 3'- -UAGCaACGGCAGc-AAAGGAGGa-GAuc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 44126 | 0.68 | 0.984699 |
Target: 5'- aGUCG--GCCGUCGcugcUCCUCCUCc-- -3' miRNA: 3'- -UAGCaaCGGCAGCaa--AGGAGGAGauc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 223356 | 0.68 | 0.982836 |
Target: 5'- -cCGgUGCCGUCGUcucUCCUCCgagcgCUGc -3' miRNA: 3'- uaGCaACGGCAGCAa--AGGAGGa----GAUc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 223772 | 0.68 | 0.979506 |
Target: 5'- uAUCGUcggcgcugcuggugcUGCCGUUGUcgUCgCUCCUCUu- -3' miRNA: 3'- -UAGCA---------------ACGGCAGCAa-AG-GAGGAGAuc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 94166 | 0.68 | 0.978603 |
Target: 5'- uUCGUUGCCG-----UCCUCCUCg-- -3' miRNA: 3'- uAGCAACGGCagcaaAGGAGGAGauc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 94345 | 0.69 | 0.976217 |
Target: 5'- aAUCGacGUCGUCG--UCCUCCUCgAGg -3' miRNA: 3'- -UAGCaaCGGCAGCaaAGGAGGAGaUC- -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 239508 | 0.69 | 0.976217 |
Target: 5'- cAUgGgaGCCGUCGUgucgcuaUCUUCCUCUGu -3' miRNA: 3'- -UAgCaaCGGCAGCAa------AGGAGGAGAUc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 142510 | 0.69 | 0.973643 |
Target: 5'- cGUCGUgGCCGcCGccaccacUUCCUCCUCUu- -3' miRNA: 3'- -UAGCAaCGGCaGCa------AAGGAGGAGAuc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 181826 | 0.69 | 0.964715 |
Target: 5'- -cCGUaGCCuucGUCGUcccCCUCCUCUAGc -3' miRNA: 3'- uaGCAaCGG---CAGCAaa-GGAGGAGAUC- -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 197218 | 0.7 | 0.957698 |
Target: 5'- cUCGUUGUCGUCGcgcucgUCgUCCUCg-- -3' miRNA: 3'- uAGCAACGGCAGCaa----AGgAGGAGauc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 27729 | 0.72 | 0.889349 |
Target: 5'- cGUCGa-GCCG-CGUcUCCUCCUCUAu -3' miRNA: 3'- -UAGCaaCGGCaGCAaAGGAGGAGAUc -5' |
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14111 | 3' | -51.9 | NC_003521.1 | + | 160336 | 0.76 | 0.716491 |
Target: 5'- uGUgGUUGUCGUCGUUaucgucgucgUCCUCCUCUu- -3' miRNA: 3'- -UAgCAACGGCAGCAA----------AGGAGGAGAuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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