Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 144 | 3' | -59.3 | AC_000006.1 | + | 20999 | 1.1 | 0.000211 |
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Target: 5'- uCGUGCACUGGCCCGACCGACCCAUGGa -3' miRNA: 3'- -GCACGUGACCGGGCUGGCUGGGUACC- -5' |
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| 144 | 3' | -59.3 | AC_000006.1 | + | 8269 | 0.73 | 0.133947 |
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Target: 5'- -cUGCGCggGGCCaCGACCGugCCGcGGu -3' miRNA: 3'- gcACGUGa-CCGG-GCUGGCugGGUaCC- -5' |
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| 144 | 3' | -59.3 | AC_000006.1 | + | 20940 | 0.72 | 0.171405 |
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Target: 5'- cCGUGCAggGGCCCGACuCGccGCCUgcGGa -3' miRNA: 3'- -GCACGUgaCCGGGCUG-GC--UGGGuaCC- -5' |
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| 144 | 3' | -59.3 | AC_000006.1 | + | 15611 | 0.7 | 0.206748 |
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Target: 5'- gCGUGCGC-GuGCCCGugCGcACCCGUc- -3' miRNA: 3'- -GCACGUGaC-CGGGCugGC-UGGGUAcc -5' |
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| 144 | 3' | -59.3 | AC_000006.1 | + | 20757 | 0.69 | 0.254677 |
|
Target: 5'- gCGUGCACUGGCUCGccuucgGCUGgaACCCGc-- -3' miRNA: 3'- -GCACGUGACCGGGC------UGGC--UGGGUacc -5' |
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| 144 | 3' | -59.3 | AC_000006.1 | + | 22647 | 0.68 | 0.303775 |
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Target: 5'- uCGUGCuCcGGCggauagCGcGCCGACCCGUGGc -3' miRNA: 3'- -GCACGuGaCCGg-----GC-UGGCUGGGUACC- -5' |
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| 144 | 3' | -59.3 | AC_000006.1 | + | 15869 | 0.67 | 0.343105 |
|
Target: 5'- gGUGCACgcguguugcGGCCCGGCaCGG-CgGUGGu -3' miRNA: 3'- gCACGUGa--------CCGGGCUG-GCUgGgUACC- -5' |
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| 144 | 3' | -59.3 | AC_000006.1 | + | 23599 | 0.66 | 0.385974 |
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Target: 5'- --cGCGCUGGCUugguacccgCGACCcccaGGCCC-UGGa -3' miRNA: 3'- gcaCGUGACCGG---------GCUGG----CUGGGuACC- -5' |
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| 144 | 3' | -59.3 | AC_000006.1 | + | 15507 | 0.72 | 0.176102 |
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Target: 5'- --cGCAUcaUGGCCCGG-CGGCCCGUGc -3' miRNA: 3'- gcaCGUG--ACCGGGCUgGCUGGGUACc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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