Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 144 | 5' | -54 | AC_000006.1 | + | 17068 | 0.66 | 0.666735 |
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Target: 5'- gCACCA---GCcaGCUGaACGGGGGcGCc -3' miRNA: 3'- -GUGGUacuUGaaCGAC-UGCCCCCaCG- -5' |
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| 144 | 5' | -54 | AC_000006.1 | + | 22346 | 0.67 | 0.597773 |
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Target: 5'- aUACCAUGAACgUGCUGAUGGaacGUuaccGCg -3' miRNA: 3'- -GUGGUACUUGaACGACUGCCcc-CA----CG- -5' |
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| 144 | 5' | -54 | AC_000006.1 | + | 12366 | 0.68 | 0.529921 |
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Target: 5'- aACC-UGcGCggGCUGugGGGcGUGCa -3' miRNA: 3'- gUGGuACuUGaaCGACugCCCcCACG- -5' |
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| 144 | 5' | -54 | AC_000006.1 | + | 23523 | 0.69 | 0.475618 |
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Target: 5'- gACCuUGGGCgggagGUUGACGGcGGGguagGCg -3' miRNA: 3'- gUGGuACUUGaa---CGACUGCC-CCCa---CG- -5' |
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| 144 | 5' | -54 | AC_000006.1 | + | 20413 | 0.69 | 0.444395 |
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Target: 5'- --aCAUGAAgUUGCUGGagaaGGGGaUGCg -3' miRNA: 3'- gugGUACUUgAACGACUg---CCCCcACG- -5' |
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| 144 | 5' | -54 | AC_000006.1 | + | 9829 | 0.71 | 0.367173 |
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Target: 5'- gGCCAUGAGCgaccaGUUGACGGucugcaggccGGGcUGCa -3' miRNA: 3'- gUGGUACUUGaa---CGACUGCC----------CCC-ACG- -5' |
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| 144 | 5' | -54 | AC_000006.1 | + | 26990 | 0.71 | 0.344141 |
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Target: 5'- aACCAUGAAggggcuCUUGCUGAUuauccuuucccugguGgGGGGUGUa -3' miRNA: 3'- gUGGUACUU------GAACGACUG---------------C-CCCCACG- -5' |
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| 144 | 5' | -54 | AC_000006.1 | + | 23692 | 0.72 | 0.32052 |
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Target: 5'- cCAgCAUGAGCUUGCgccgcugcuccagGGCcuGGGGGUcGCg -3' miRNA: 3'- -GUgGUACUUGAACGa------------CUG--CCCCCA-CG- -5' |
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| 144 | 5' | -54 | AC_000006.1 | + | 21035 | 1.11 | 0.000531 |
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Target: 5'- cCACCAUGAACUUGCUGACGGGGGUGCc -3' miRNA: 3'- -GUGGUACUUGAACGACUGCCCCCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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