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miRNA: 3'- aUgGUGGACCGGG----UCUACCUCU-UGaa -5'
miRNA: 3'- aUGGU-GGaCCGGGUCuACCUCUUGAa -5'
miRNA: 3'- -AUGGUGGaCCGGG-----UCUACCUCUUGaa -5'
miRNA: 3'- aUGGUGGACCGGguCUAC-CUCuUGAa -5'
miRNA: 3'- -AUGGUGGACCGGGUCUACCUCUUGAA- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 14464 | 5' | -56.3 | NC_003521.1 | + | 36702 | 0.72 | 0.658811 |
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Target: 5'- cGgCGCCUGGCCCgagcAGGUGGAGGcGCa- -3' miRNA: 3'- aUgGUGGACCGGG----UCUACCUCU-UGaa -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 228898 | 0.75 | 0.461371 |
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Target: 5'- cACCAgCCgGGCCCGGcUGGGGGACUc -3' miRNA: 3'- aUGGU-GGaCCGGGUCuACCUCUUGAa -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 64669 | 0.76 | 0.434192 |
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Target: 5'- cUGCCACCgGGCUCcgaggAGAUGGAGAACc- -3' miRNA: 3'- -AUGGUGGaCCGGG-----UCUACCUCUUGaa -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 73560 | 0.76 | 0.399445 |
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Target: 5'- aACCACCUGGCCguGGUGcGGGcGCUg -3' miRNA: 3'- aUGGUGGACCGGguCUAC-CUCuUGAa -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 112655 | 1.03 | 0.008268 |
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Target: 5'- cUACCACCUGGCCCAGAUGGAGAACUUc -3' miRNA: 3'- -AUGGUGGACCGGGUCUACCUCUUGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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