miRNA: 3'- aUGGUGGACCgggUCUACCUCUUGaa -5'
miRNA: 3'- auGGUGGACCgGGUCUACC-UCU---UGAA- -5'
miRNA: 3'- -AUG-GUGGa-CCGGGUCUa---CCUCUUGaa -5'
miRNA: 3'- aUG-GUG----GACCG-GGUCUACCUCUUGaa -5'
miRNA: 3'- auGGUGGACCgGGUCUACcUCUUGaa -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14464 | 5' | -56.3 | NC_003521.1 | + | 16890 | 0.7 | 0.757707 |
|
Target: 5'- gGCCAUCUGGaggAGGUGGAGGACg- -3' miRNA: 3'- aUGGUGGACCgggUCUACCUCUUGaa -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 128173 | 0.7 | 0.767143 |
|
Target: 5'- -uCCAUCUGGgCCAGGUGGuAGAcgcGCUUc -3' miRNA: 3'- auGGUGGACCgGGUCUACC-UCU---UGAA- -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 236324 | 0.69 | 0.77646 |
|
Target: 5'- uUACuCAUCggGGCCCGGAcgggGGAGGACg- -3' miRNA: 3'- -AUG-GUGGa-CCGGGUCUa---CCUCUUGaa -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 117397 | 0.69 | 0.77646 |
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Target: 5'- cACgCGCugugCUGGCaCCGGGUGGAGGGCg- -3' miRNA: 3'- aUG-GUG----GACCG-GGUCUACCUCUUGaa -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 128201 | 0.66 | 0.936181 |
|
Target: 5'- -uCCACCUGGgCUGGGUGuAGAGCc- -3' miRNA: 3'- auGGUGGACCgGGUCUACcUCUUGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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