miRNA: 3'- gUUCUCGG-ACGUGCa---CA-CGugGUGAu -5'
miRNA: 3'- gUUCUCgGACGUGcACACGUGGUGau -5'
miRNA: 3'- guUCUCGgaCGUgCACa-CGUGGUGau -5'
miRNA: 3'- guucUCGGa--CGUGCA---CACGUGGUGau -5'
miRNA: 3'- -GUUCUCG--GACGUgCacaCGUGGUGau -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14528 | 3' | -55 | NC_003521.1 | + | 167471 | 0.65 | 0.967796 |
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Target: 5'- uGAGGGCCgUGCGCGgcgcGUaGUugCACUu -3' miRNA: 3'- gUUCUCGG-ACGUGCa---CA-CGugGUGAu -5' |
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| 14528 | 3' | -55 | NC_003521.1 | + | 11990 | 0.65 | 0.967796 |
|
Target: 5'- aGGGAG-CUGUAUcUGUGUACCGCa- -3' miRNA: 3'- gUUCUCgGACGUGcACACGUGGUGau -5' |
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| 14528 | 3' | -55 | NC_003521.1 | + | 167906 | 0.65 | 0.967796 |
|
Target: 5'- -uAGAGCg-GCAgGUGggGCGCCGCg- -3' miRNA: 3'- guUCUCGgaCGUgCACa-CGUGGUGau -5' |
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| 14528 | 3' | -55 | NC_003521.1 | + | 148842 | 0.65 | 0.967796 |
|
Target: 5'- ---cGGCCgagGUGCGUgccGUGCACCGCg- -3' miRNA: 3'- guucUCGGa--CGUGCA---CACGUGGUGau -5' |
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| 14528 | 3' | -55 | NC_003521.1 | + | 226880 | 0.65 | 0.967796 |
|
Target: 5'- gCGAGGGCagCUGCGgGg--GCACCACg- -3' miRNA: 3'- -GUUCUCG--GACGUgCacaCGUGGUGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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