Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14605 | 5' | -57 | NC_003521.1 | + | 67521 | 0.66 | 0.938575 |
Target: 5'- gUCGCUgagcaaGCUGUUcUGGgacuCGGAGAUCAUg -3' miRNA: 3'- -AGCGAg-----CGGCAGuACC----GCCUCUAGUA- -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 6023 | 0.66 | 0.937177 |
Target: 5'- aUCGCugUCGUCGUCGUaccacucgcucucgGGCGGuagcGGGUCGa -3' miRNA: 3'- -AGCG--AGCGGCAGUA--------------CCGCC----UCUAGUa -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 178948 | 0.66 | 0.933838 |
Target: 5'- cUGCUCGCUaucCGUGGUGGucGUCAUg -3' miRNA: 3'- aGCGAGCGGca-GUACCGCCucUAGUA- -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 135905 | 0.66 | 0.933352 |
Target: 5'- gCGCUCGUCGUCAUcgucgucGGaCGGcagcaggcugGGAUCGa -3' miRNA: 3'- aGCGAGCGGCAGUA-------CC-GCC----------UCUAGUa -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 38912 | 0.66 | 0.928876 |
Target: 5'- gCGUcccCGCCcuguGUCGUGGCGGcaguAGAUCGUu -3' miRNA: 3'- aGCGa--GCGG----CAGUACCGCC----UCUAGUA- -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 167590 | 0.67 | 0.912642 |
Target: 5'- cCGCUgcuguUGCCGcUCGUGGUGGcguuGGUCGUc -3' miRNA: 3'- aGCGA-----GCGGC-AGUACCGCCu---CUAGUA- -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 21755 | 0.67 | 0.903162 |
Target: 5'- cUCGCUgcucaugcaccugggCGCCGUggcgugccugucCGUGGCGGuGAUCc- -3' miRNA: 3'- -AGCGA---------------GCGGCA------------GUACCGCCuCUAGua -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 169563 | 0.67 | 0.894405 |
Target: 5'- cCGCcucCGCCGaUguUGGCGG-GAUCGUg -3' miRNA: 3'- aGCGa--GCGGC-AguACCGCCuCUAGUA- -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 74073 | 0.67 | 0.881165 |
Target: 5'- gCGC-CGCUGUC-UGGCGGgcgAGGUCu- -3' miRNA: 3'- aGCGaGCGGCAGuACCGCC---UCUAGua -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 94181 | 0.68 | 0.874229 |
Target: 5'- cUCGC-CGUCGUCGUGGcCGGGGc---- -3' miRNA: 3'- -AGCGaGCGGCAGUACC-GCCUCuagua -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 33041 | 0.68 | 0.867091 |
Target: 5'- -aGCUguaGCCGUCGUccgggggagaGGCGGAGAcaUCGUc -3' miRNA: 3'- agCGAg--CGGCAGUA----------CCGCCUCU--AGUA- -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 140807 | 0.68 | 0.859753 |
Target: 5'- cUGCUgCGUCGUCugGUGGUGGAGAa--- -3' miRNA: 3'- aGCGA-GCGGCAG--UACCGCCUCUagua -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 23566 | 0.68 | 0.852222 |
Target: 5'- cUC-CUCGuCCGUCAUGGUGGuGggCGc -3' miRNA: 3'- -AGcGAGC-GGCAGUACCGCCuCuaGUa -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 135356 | 0.68 | 0.852222 |
Target: 5'- cCGCgcgCGCCGUCuccgcGGCGGuuAUCGc -3' miRNA: 3'- aGCGa--GCGGCAGua---CCGCCucUAGUa -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 148915 | 0.68 | 0.836603 |
Target: 5'- cCGC-CGCCGUCcUGGaucCGGGGGUCc- -3' miRNA: 3'- aGCGaGCGGCAGuACC---GCCUCUAGua -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 153946 | 0.69 | 0.820284 |
Target: 5'- cCGCcgCGCCGUCAUGGUGucGAaCAg -3' miRNA: 3'- aGCGa-GCGGCAGUACCGCcuCUaGUa -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 5678 | 0.69 | 0.820284 |
Target: 5'- gCGCUCGgaGUcCGUGGUGGcGAUCAc -3' miRNA: 3'- aGCGAGCggCA-GUACCGCCuCUAGUa -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 207718 | 0.69 | 0.81188 |
Target: 5'- cCGCUaCGCCGUCAucggcUGGCuGGAGccCGUg -3' miRNA: 3'- aGCGA-GCGGCAGU-----ACCG-CCUCuaGUA- -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 164954 | 0.69 | 0.79198 |
Target: 5'- cUCGCgUCGCCGgagagggcccucuuUCAUGGaggacggacUGGAGAUCAUc -3' miRNA: 3'- -AGCG-AGCGGC--------------AGUACC---------GCCUCUAGUA- -5' |
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14605 | 5' | -57 | NC_003521.1 | + | 166988 | 0.7 | 0.785777 |
Target: 5'- -gGUUCGCgGUCAgGGCGGAGuuggCGUa -3' miRNA: 3'- agCGAGCGgCAGUaCCGCCUCua--GUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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