Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14605 | 5' | -57 | NC_003521.1 | + | 237392 | 0.7 | 0.749193 |
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Target: 5'- cUCGg-CGUCGgCAUGGCGGGGGUCu- -3' miRNA: 3'- -AGCgaGCGGCaGUACCGCCUCUAGua -5' |
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| 14605 | 5' | -57 | NC_003521.1 | + | 135731 | 0.71 | 0.730286 |
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Target: 5'- gCGCUCGCCGcugcuacugcggUgGUGGCGGcggugGGAUCGc -3' miRNA: 3'- aGCGAGCGGC------------AgUACCGCC-----UCUAGUa -5' |
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| 14605 | 5' | -57 | NC_003521.1 | + | 211712 | 0.73 | 0.602527 |
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Target: 5'- -aGCUUGCCGcCGUGGCGGA--UCAc -3' miRNA: 3'- agCGAGCGGCaGUACCGCCUcuAGUa -5' |
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| 14605 | 5' | -57 | NC_003521.1 | + | 98895 | 0.74 | 0.54374 |
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Target: 5'- gCGC-CGUCGUCGcGGCGGAGAaCAUg -3' miRNA: 3'- aGCGaGCGGCAGUaCCGCCUCUaGUA- -5' |
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| 14605 | 5' | -57 | NC_003521.1 | + | 25691 | 0.74 | 0.524533 |
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Target: 5'- aCGCUgGCCuUCGUGGCGGcccuGAUCGUc -3' miRNA: 3'- aGCGAgCGGcAGUACCGCCu---CUAGUA- -5' |
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| 14605 | 5' | -57 | NC_003521.1 | + | 217559 | 0.94 | 0.037468 |
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Target: 5'- aCGUcgUCGCCGUCAUGGCGGAGAUCAUc -3' miRNA: 3'- aGCG--AGCGGCAGUACCGCCUCUAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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