Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14605 | 5' | -57 | NC_003521.1 | + | 167590 | 0.67 | 0.912642 |
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Target: 5'- cCGCUgcuguUGCCGcUCGUGGUGGcguuGGUCGUc -3' miRNA: 3'- aGCGA-----GCGGC-AGUACCGCCu---CUAGUA- -5' |
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| 14605 | 5' | -57 | NC_003521.1 | + | 38912 | 0.66 | 0.928876 |
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Target: 5'- gCGUcccCGCCcuguGUCGUGGCGGcaguAGAUCGUu -3' miRNA: 3'- aGCGa--GCGG----CAGUACCGCC----UCUAGUA- -5' |
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| 14605 | 5' | -57 | NC_003521.1 | + | 135905 | 0.66 | 0.933352 |
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Target: 5'- gCGCUCGUCGUCAUcgucgucGGaCGGcagcaggcugGGAUCGa -3' miRNA: 3'- aGCGAGCGGCAGUA-------CC-GCC----------UCUAGUa -5' |
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| 14605 | 5' | -57 | NC_003521.1 | + | 178948 | 0.66 | 0.933838 |
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Target: 5'- cUGCUCGCUaucCGUGGUGGucGUCAUg -3' miRNA: 3'- aGCGAGCGGca-GUACCGCCucUAGUA- -5' |
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| 14605 | 5' | -57 | NC_003521.1 | + | 6023 | 0.66 | 0.937177 |
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Target: 5'- aUCGCugUCGUCGUCGUaccacucgcucucgGGCGGuagcGGGUCGa -3' miRNA: 3'- -AGCG--AGCGGCAGUA--------------CCGCC----UCUAGUa -5' |
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| 14605 | 5' | -57 | NC_003521.1 | + | 67521 | 0.66 | 0.938575 |
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Target: 5'- gUCGCUgagcaaGCUGUUcUGGgacuCGGAGAUCAUg -3' miRNA: 3'- -AGCGAg-----CGGCAGuACC----GCCUCUAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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