Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14610 | 5' | -54.3 | NC_003521.1 | + | 154049 | 0.69 | 0.930425 |
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Target: 5'- gGUGCCCaUGGAgaucaAGCUCUCGcgccuCUCg -3' miRNA: 3'- -CGCGGG-ACCUaca--UCGAGAGCau---GAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 156637 | 0.7 | 0.875387 |
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Target: 5'- aCGCCCUGGGcuuc-CUCuUCGUGCUCg -3' miRNA: 3'- cGCGGGACCUacaucGAG-AGCAUGAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 165311 | 0.7 | 0.868167 |
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Target: 5'- -aGCuCCUGGAUG-AGCUcCUUGUagcGCUCg -3' miRNA: 3'- cgCG-GGACCUACaUCGA-GAGCA---UGAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 169942 | 0.68 | 0.948916 |
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Target: 5'- -gGCCCUGG-UGUGGCgauaugUCgUCGUuGCUCu -3' miRNA: 3'- cgCGGGACCuACAUCG------AG-AGCA-UGAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 172560 | 0.67 | 0.963812 |
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Target: 5'- aUGCUggGGAUGUugaagggcaGGUUCUUGUACUCc -3' miRNA: 3'- cGCGGgaCCUACA---------UCGAGAGCAUGAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 175246 | 0.69 | 0.911798 |
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Target: 5'- uGCuGuCCCUGGcgGUAGCgugcguaguccugCUCGUAgUCg -3' miRNA: 3'- -CG-C-GGGACCuaCAUCGa------------GAGCAUgAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 212517 | 0.68 | 0.939201 |
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Target: 5'- cGCGCCg-GGGUcgcugccgcuGCUCUCGUugUCg -3' miRNA: 3'- -CGCGGgaCCUAcau-------CGAGAGCAugAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 213117 | 0.66 | 0.975368 |
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Target: 5'- aCGCgCUGGGUGUGGCg-UgGUGCg- -3' miRNA: 3'- cGCGgGACCUACAUCGagAgCAUGag -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 220793 | 1.12 | 0.004008 |
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Target: 5'- gGCGCCCUGGAUGUAGCUCUCGUACUCg -3' miRNA: 3'- -CGCGGGACCUACAUCGAGAGCAUGAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 227624 | 0.66 | 0.980009 |
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Target: 5'- uCGUCCUGGGUGaAGUUggCGUGCa- -3' miRNA: 3'- cGCGGGACCUACaUCGAgaGCAUGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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