Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14633 | 5' | -52.1 | NC_003521.1 | + | 172113 | 0.68 | 0.981669 |
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Target: 5'- gGCGGCCGACggCGCC---AcgGUCaUGa -3' miRNA: 3'- -UGCCGGCUGa-GCGGcuuUuaCAGaAC- -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 172290 | 0.71 | 0.938578 |
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Target: 5'- gGCGGCCGcuGC-CGCCGuccGGUGUCggUGg -3' miRNA: 3'- -UGCCGGC--UGaGCGGCuu-UUACAGa-AC- -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 182179 | 0.67 | 0.992208 |
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Target: 5'- gACGGCCGGCgCGUgGGGAuucagacacuugGUGcUCUUGu -3' miRNA: 3'- -UGCCGGCUGaGCGgCUUU------------UAC-AGAAC- -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 197385 | 0.67 | 0.991104 |
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Target: 5'- -aGGCUcuGGCUCGCCGcccgggacuGGGUGUCUg- -3' miRNA: 3'- ugCCGG--CUGAGCGGCu--------UUUACAGAac -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 206758 | 0.66 | 0.995588 |
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Target: 5'- uGCcGCCGACuaUCGCCGAuuAUcGUCg-- -3' miRNA: 3'- -UGcCGGCUG--AGCGGCUuuUA-CAGaac -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 211585 | 0.67 | 0.99156 |
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Target: 5'- cGCGGCgGugUCcCCGGAgacguccugcagcggGAUGUCUUc -3' miRNA: 3'- -UGCCGgCugAGcGGCUU---------------UUACAGAAc -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 214731 | 0.66 | 0.996211 |
|
Target: 5'- cUGGCCGACU--CCGGAGccGUCUa- -3' miRNA: 3'- uGCCGGCUGAgcGGCUUUuaCAGAac -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 229099 | 1.09 | 0.010905 |
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Target: 5'- gACGGCCGACUCGCCGAAAAUGUCUUGa -3' miRNA: 3'- -UGCCGGCUGAGCGGCUUUUACAGAAC- -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 234453 | 0.66 | 0.996211 |
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Target: 5'- -gGGCCGACcucCGCUcuGAGUGUCUc- -3' miRNA: 3'- ugCCGGCUGa--GCGGcuUUUACAGAac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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