miRNA: 3'- cUCCUCCUGgU-CgGUGGA--CGGUUCUu -5'
miRNA: 3'- cUCCU------------CCUGGUCGG--UGGACGGuUCuu -5'
miRNA: 3'- cuCC-UCCUGgUCG-GUGG-ACG-GUUCUU- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14641 | 5' | -58.6 | NC_003521.1 | + | 99952 | 0.66 | 0.862858 |
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Target: 5'- cAGGGGGGCgGcGgCGCCUcgGCCGAGAc -3' miRNA: 3'- cUCCUCCUGgU-CgGUGGA--CGGUUCUu -5' |
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| 14641 | 5' | -58.6 | NC_003521.1 | + | 112285 | 0.66 | 0.862858 |
|
Target: 5'- aAGGGccaccugacgucGGGCCAGCCgcACCUGCCcAGc- -3' miRNA: 3'- cUCCU------------CCUGGUCGG--UGGACGGuUCuu -5' |
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| 14641 | 5' | -58.6 | NC_003521.1 | + | 94125 | 0.66 | 0.870159 |
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Target: 5'- -cGGaAGGACgAGCgCACCgUGCuCAGGGAg -3' miRNA: 3'- cuCC-UCCUGgUCG-GUGG-ACG-GUUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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