Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14641 | 5' | -58.6 | NC_003521.1 | + | 228892 | 0.66 | 0.855359 |
Target: 5'- cAGGAGcACCAGCCggGCCcgGCUggGGGa -3' miRNA: 3'- cUCCUCcUGGUCGG--UGGa-CGGuuCUU- -5' |
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14641 | 5' | -58.6 | NC_003521.1 | + | 236501 | 1.05 | 0.003538 |
Target: 5'- cGAGGAGGACCAGCCACCUGCCAAGAGa -3' miRNA: 3'- -CUCCUCCUGGUCGGUGGACGGUUCUU- -5' |
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14641 | 5' | -58.6 | NC_003521.1 | + | 238187 | 0.69 | 0.674728 |
Target: 5'- cAGGAGGAUCauAGCCAagUCgagGCCAAGAGc -3' miRNA: 3'- cUCCUCCUGG--UCGGU--GGa--CGGUUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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