Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14643 | 3' | -52.1 | NC_003521.1 | + | 155282 | 0.67 | 0.991977 |
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Target: 5'- -aCGCCUCUGAGAugaCGCGgcUGUuccUCUCg -3' miRNA: 3'- caGUGGGGACUUUug-GCGC--ACA---AGAG- -5' |
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| 14643 | 3' | -52.1 | NC_003521.1 | + | 66006 | 0.66 | 0.993 |
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Target: 5'- aGUCGCUgCUGGAGAaccCCGUGUGggCg- -3' miRNA: 3'- -CAGUGGgGACUUUU---GGCGCACaaGag -5' |
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| 14643 | 3' | -52.1 | NC_003521.1 | + | 175503 | 0.66 | 0.995458 |
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Target: 5'- aUC-UCUCUGGugaaguGCCGCGUGUggUCUCu -3' miRNA: 3'- cAGuGGGGACUuu----UGGCGCACA--AGAG- -5' |
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| 14643 | 3' | -52.1 | NC_003521.1 | + | 131446 | 0.66 | 0.995458 |
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Target: 5'- gGUCAUCaacaUGAAGGCCGCGc--UCUCg -3' miRNA: 3'- -CAGUGGgg--ACUUUUGGCGCacaAGAG- -5' |
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| 14643 | 3' | -52.1 | NC_003521.1 | + | 68442 | 0.66 | 0.996665 |
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Target: 5'- -gCGCCCCUc--AACCGCGUGccggUCa- -3' miRNA: 3'- caGUGGGGAcuuUUGGCGCACa---AGag -5' |
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| 14643 | 3' | -52.1 | NC_003521.1 | + | 240324 | 1.1 | 0.008858 |
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Target: 5'- cGUCACCCCUGAAAACCGCGUGUUCUCu -3' miRNA: 3'- -CAGUGGGGACUUUUGGCGCACAAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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