Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14703 | 5' | -50 | NC_003529.1 | + | 66520 | 0.66 | 0.998392 |
Target: 5'- uUGAUCGUuuGCUugucgacGAGCAGAACauuguguuCCa -3' miRNA: 3'- gGCUAGUAggCGA-------CUCGUUUUGgu------GG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 40538 | 0.66 | 0.998332 |
Target: 5'- aCCGAUCcuuucuguggcgacAUCaUGUUGAGCAcucgcAGCCACg -3' miRNA: 3'- -GGCUAG--------------UAG-GCGACUCGUu----UUGGUGg -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 30297 | 0.66 | 0.998106 |
Target: 5'- gCCGG-CAUUCGCaGAGCGGucAACuCACg -3' miRNA: 3'- -GGCUaGUAGGCGaCUCGUU--UUG-GUGg -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 37869 | 0.66 | 0.998106 |
Target: 5'- cCCGAUCAcgguuUgCGCgUGcGCGAAAUgGCCc -3' miRNA: 3'- -GGCUAGU-----AgGCG-ACuCGUUUUGgUGG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 114984 | 0.66 | 0.998106 |
Target: 5'- aCGGUCGagaCG-UGAGCAAuguucgcgcuugGACCACCc -3' miRNA: 3'- gGCUAGUag-GCgACUCGUU------------UUGGUGG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 148526 | 0.66 | 0.998106 |
Target: 5'- aCGuUCGUagCCGCUGAcCAAAcgcguuccGCCGCCc -3' miRNA: 3'- gGCuAGUA--GGCGACUcGUUU--------UGGUGG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 20544 | 0.66 | 0.997739 |
Target: 5'- gUGAUaauccuUUCGC-GGGCAcgGCCACCg -3' miRNA: 3'- gGCUAgu----AGGCGaCUCGUuuUGGUGG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 13391 | 0.66 | 0.997314 |
Target: 5'- cCCGAUCAUCguCGCcGuucuCAAuaauGACCACCu -3' miRNA: 3'- -GGCUAGUAG--GCGaCuc--GUU----UUGGUGG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 46345 | 0.66 | 0.997314 |
Target: 5'- cCCGAUgCGUCgGUaGGGCGGuuCUACUg -3' miRNA: 3'- -GGCUA-GUAGgCGaCUCGUUuuGGUGG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 115380 | 0.66 | 0.997222 |
Target: 5'- aCCGAga---UGCUGAGCcgcauauuaauGGCCACCa -3' miRNA: 3'- -GGCUaguagGCGACUCGuu---------UUGGUGG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 58111 | 0.67 | 0.996265 |
Target: 5'- gCCGAgaUCGUCgCGCUc--CGAAugCACCa -3' miRNA: 3'- -GGCU--AGUAG-GCGAcucGUUUugGUGG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 153443 | 0.67 | 0.995625 |
Target: 5'- uUGcgCAUaCCGUacgUGAGCGAuGCUGCCg -3' miRNA: 3'- gGCuaGUA-GGCG---ACUCGUUuUGGUGG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 83101 | 0.68 | 0.99212 |
Target: 5'- cUCGAUCuuuguggugCCGUgcaauUGGGCAcacGCCACCg -3' miRNA: 3'- -GGCUAGua-------GGCG-----ACUCGUuu-UGGUGG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 59365 | 0.68 | 0.99212 |
Target: 5'- uCgGGUCGaCCGgUGAGCGAAGCguCACg -3' miRNA: 3'- -GgCUAGUaGGCgACUCGUUUUG--GUGg -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 27988 | 0.68 | 0.989679 |
Target: 5'- gCCGGUCuuUCUcaGCUcGGGCAAAACUuCCu -3' miRNA: 3'- -GGCUAGu-AGG--CGA-CUCGUUUUGGuGG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 3845 | 0.68 | 0.988255 |
Target: 5'- gCGAU-GUCUGCUGuAGUAGAAuCCGCUu -3' miRNA: 3'- gGCUAgUAGGCGAC-UCGUUUU-GGUGG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 112689 | 0.69 | 0.984955 |
Target: 5'- -aGAUCGUuuGCaa--CAAAGCCACCg -3' miRNA: 3'- ggCUAGUAggCGacucGUUUUGGUGG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 52116 | 0.69 | 0.98306 |
Target: 5'- aCGAU-GUCUGCUGuacGCAAAACCuuguACCc -3' miRNA: 3'- gGCUAgUAGGCGACu--CGUUUUGG----UGG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 119903 | 0.69 | 0.98306 |
Target: 5'- gCGGUCGaCCGCcugccgugccaUGuGUAAAGCCACUu -3' miRNA: 3'- gGCUAGUaGGCG-----------ACuCGUUUUGGUGG- -5' |
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14703 | 5' | -50 | NC_003529.1 | + | 29965 | 0.69 | 0.976295 |
Target: 5'- aCUGucUCGUUCGgUGAGC-GGACCGCUg -3' miRNA: 3'- -GGCu-AGUAGGCgACUCGuUUUGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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