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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 14819 | 5' | -59.3 | NC_003607.1 | + | 2196 | 0.66 | 0.07535 |
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Target: 5'- aCCCucacuagugCCCCUgCGCCGAuuCCGGCua-GCCCg -3' miRNA: 3'- -GGG---------GGGGA-GCGGUU--GGUUGuggUGGG- -5' |
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| 14819 | 5' | -59.3 | NC_003607.1 | + | 1522 | 0.67 | 0.065729 |
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Target: 5'- gUCgCCCCUUacuuuuggauugaGCCGACCGGCuugauCC-CCCa -3' miRNA: 3'- -GGgGGGGAG-------------CGGUUGGUUGu----GGuGGG- -5' |
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| 14819 | 5' | -59.3 | NC_003607.1 | + | 990 | 0.68 | 0.047139 |
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Target: 5'- gCCCCCUCUUGCUcuuGCCc---UCACCCg -3' miRNA: 3'- -GGGGGGGAGCGGu--UGGuuguGGUGGG- -5' |
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| 14819 | 5' | -59.3 | NC_003607.1 | + | 2465 | 0.71 | 0.028341 |
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Target: 5'- uCCaCCCCCUgGCCcACCAAacggucccccUGCCgGCCCa -3' miRNA: 3'- -GG-GGGGGAgCGGuUGGUU----------GUGG-UGGG- -5' |
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| 14819 | 5' | -59.3 | NC_003607.1 | + | 2538 | 0.71 | 0.029323 |
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Target: 5'- gCCCCCgUCcCCAcuGCCAuccacGCACCcCCCg -3' miRNA: 3'- gGGGGGgAGcGGU--UGGU-----UGUGGuGGG- -5' |
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| 14819 | 5' | -59.3 | NC_003607.1 | + | 4614 | 0.72 | 0.022626 |
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Target: 5'- gUCCCCCCUggugccgguaugcaCGCUAccaugucauaccuaGCCAgacACACCACCUu -3' miRNA: 3'- -GGGGGGGA--------------GCGGU--------------UGGU---UGUGGUGGG- -5' |
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| 14819 | 5' | -59.3 | NC_003607.1 | + | 4862 | 0.74 | 0.014446 |
|
Target: 5'- aCCCCUCUgccggucaguucgugUGCCAuagagaaGCCGACAgCCACCCu -3' miRNA: 3'- gGGGGGGA---------------GCGGU-------UGGUUGU-GGUGGG- -5' |
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| 14819 | 5' | -59.3 | NC_003607.1 | + | 214 | 1.09 | 0.000012 |
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Target: 5'- gCCCCCCCUCGCCAACCAACACCACCUu -3' miRNA: 3'- -GGGGGGGAGCGGUUGGUUGUGGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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