Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14832 | 5' | -51.3 | NC_003607.1 | + | 3198 | 0.67 | 0.196643 |
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Target: 5'- cGAGGGCGUaG-CGUGgGCUAuCGCUu -3' miRNA: 3'- uCUUCCGUAaCaGCGCgUGAUuGCGG- -5' |
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| 14832 | 5' | -51.3 | NC_003607.1 | + | 1592 | 0.68 | 0.177922 |
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Target: 5'- aAGAAGGUu---UCGCGUcguacuGCUGGCGCg -3' miRNA: 3'- -UCUUCCGuaacAGCGCG------UGAUUGCGg -5' |
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| 14832 | 5' | -51.3 | NC_003607.1 | + | 1386 | 0.68 | 0.166337 |
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Target: 5'- uGggGGCAagcaacUGUUGCGCGguuuCUGucCGCCa -3' miRNA: 3'- uCuuCCGUa-----ACAGCGCGU----GAUu-GCGG- -5' |
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| 14832 | 5' | -51.3 | NC_003607.1 | + | 931 | 0.7 | 0.118029 |
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Target: 5'- -cGGGGCgAUUGUCGaCGCcCU-ACGCCu -3' miRNA: 3'- ucUUCCG-UAACAGC-GCGuGAuUGCGG- -5' |
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| 14832 | 5' | -51.3 | NC_003607.1 | + | 1058 | 0.72 | 0.080158 |
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Target: 5'- aAGggGGCAUcguccgcgaucUGUUGCGCugUGGugguuucggucUGCCu -3' miRNA: 3'- -UCuuCCGUA-----------ACAGCGCGugAUU-----------GCGG- -5' |
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| 14832 | 5' | -51.3 | NC_003607.1 | + | 3928 | 1.12 | 4.4e-05 |
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Target: 5'- gAGAAGGCAUUGUCGCGCACUAACGCCc -3' miRNA: 3'- -UCUUCCGUAACAGCGCGUGAUUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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