Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1492 | 5' | -63.1 | NC_001335.1 | + | 4929 | 0.66 | 0.30643 |
Target: 5'- cCGaCGAGCa--UCACCGGCuugaucggcaaGGCCGCUGg -3' miRNA: 3'- -GC-GCUCGacaGGUGGCCG-----------UCGGCGGC- -5' |
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1492 | 5' | -63.1 | NC_001335.1 | + | 4175 | 0.72 | 0.122934 |
Target: 5'- gGcCGAGCUGcuuugcaucgCCGCCGGCuGCCucGCCGg -3' miRNA: 3'- gC-GCUCGACa---------GGUGGCCGuCGG--CGGC- -5' |
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1492 | 5' | -63.1 | NC_001335.1 | + | 2329 | 0.69 | 0.191784 |
Target: 5'- aGUGAGCUGg--GCCGGCucaGGCCGgCGa -3' miRNA: 3'- gCGCUCGACaggUGGCCG---UCGGCgGC- -5' |
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1492 | 5' | -63.1 | NC_001335.1 | + | 1346 | 0.67 | 0.299264 |
Target: 5'- --gGGGCUGcugccaccgCCGCCGGCuccacuuccGCCGCCa -3' miRNA: 3'- gcgCUCGACa--------GGUGGCCGu--------CGGCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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