Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1495 | 3' | -58.1 | NC_001335.1 | + | 32173 | 0.66 | 0.535371 |
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Target: 5'- -gCCAGCUC-GUCGGCAUCGAagucGCc -3' miRNA: 3'- gaGGUCGAGcCAGUCGUGGCUgga-CG- -5' |
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| 1495 | 3' | -58.1 | NC_001335.1 | + | 32702 | 0.66 | 0.545909 |
|
Target: 5'- gCUCCGGU--GGaUCcGCGCCGGCCaacgGCg -3' miRNA: 3'- -GAGGUCGagCC-AGuCGUGGCUGGa---CG- -5' |
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| 1495 | 3' | -58.1 | NC_001335.1 | + | 33496 | 0.68 | 0.425443 |
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Target: 5'- aUCCGGCgugugCGGUucaaccucaaCAGCgacaugGCCGAgCUGCg -3' miRNA: 3'- gAGGUCGa----GCCA----------GUCG------UGGCUgGACG- -5' |
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| 1495 | 3' | -58.1 | NC_001335.1 | + | 35623 | 0.67 | 0.483885 |
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Target: 5'- -aCguGCUCGGUCAGC---GACUUGUg -3' miRNA: 3'- gaGguCGAGCCAGUCGuggCUGGACG- -5' |
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| 1495 | 3' | -58.1 | NC_001335.1 | + | 39617 | 0.67 | 0.463957 |
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Target: 5'- -cUCGGCaacUCaGUCGGCACCcGGCaCUGCa -3' miRNA: 3'- gaGGUCG---AGcCAGUCGUGG-CUG-GACG- -5' |
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| 1495 | 3' | -58.1 | NC_001335.1 | + | 41500 | 0.75 | 0.144367 |
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Target: 5'- -gCCAGCUaguccUGGUCuGCGCCGGCCccgGCa -3' miRNA: 3'- gaGGUCGA-----GCCAGuCGUGGCUGGa--CG- -5' |
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| 1495 | 3' | -58.1 | NC_001335.1 | + | 46364 | 0.66 | 0.523862 |
|
Target: 5'- cCUCagCGGCUaugcccaCGGUCAGCGCgaCGGCC-GCa -3' miRNA: 3'- -GAG--GUCGA-------GCCAGUCGUG--GCUGGaCG- -5' |
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| 1495 | 3' | -58.1 | NC_001335.1 | + | 48563 | 0.68 | 0.416115 |
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Target: 5'- --aCGGCUUGGUCG--ACCGGCUUGUa -3' miRNA: 3'- gagGUCGAGCCAGUcgUGGCUGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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