Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1495 | 3' | -58.1 | NC_001335.1 | + | 26734 | 0.67 | 0.477862 |
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Target: 5'- aUCCAGgagaccgcugacuacCUCGGugUCAGCACCaaGACCguccGCa -3' miRNA: 3'- gAGGUC---------------GAGCC--AGUCGUGG--CUGGa---CG- -5' |
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| 1495 | 3' | -58.1 | NC_001335.1 | + | 39617 | 0.67 | 0.463957 |
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Target: 5'- -cUCGGCaacUCaGUCGGCACCcGGCaCUGCa -3' miRNA: 3'- gaGGUCG---AGcCAGUCGUGG-CUG-GACG- -5' |
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| 1495 | 3' | -58.1 | NC_001335.1 | + | 33496 | 0.68 | 0.425443 |
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Target: 5'- aUCCGGCgugugCGGUucaaccucaaCAGCgacaugGCCGAgCUGCg -3' miRNA: 3'- gAGGUCGa----GCCA----------GUCG------UGGCUgGACG- -5' |
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| 1495 | 3' | -58.1 | NC_001335.1 | + | 6100 | 0.68 | 0.406914 |
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Target: 5'- gUCCAGCU---UCAGC-UCGAUCUGCa -3' miRNA: 3'- gAGGUCGAgccAGUCGuGGCUGGACG- -5' |
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| 1495 | 3' | -58.1 | NC_001335.1 | + | 16384 | 0.68 | 0.406914 |
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Target: 5'- uCUCCAGCgUCGGggCAGCGUCGAggcggCUGCc -3' miRNA: 3'- -GAGGUCG-AGCCa-GUCGUGGCUg----GACG- -5' |
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| 1495 | 3' | -58.1 | NC_001335.1 | + | 12729 | 0.7 | 0.34132 |
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Target: 5'- gUCCAGgUCGGggauccggcgcuugaUCAuGCGCuCGAUCUGCu -3' miRNA: 3'- gAGGUCgAGCC---------------AGU-CGUG-GCUGGACG- -5' |
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| 1495 | 3' | -58.1 | NC_001335.1 | + | 28785 | 0.72 | 0.257926 |
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Target: 5'- gUCCGGUgcUGGUUGGCACCGACCc-- -3' miRNA: 3'- gAGGUCGa-GCCAGUCGUGGCUGGacg -5' |
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| 1495 | 3' | -58.1 | NC_001335.1 | + | 26656 | 0.66 | 0.567176 |
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Target: 5'- gUUCGGCUCGGUCcccgGCUGAUCcGCg -3' miRNA: 3'- gAGGUCGAGCCAGucg-UGGCUGGaCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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