Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1506 | 5' | -65.6 | NC_001335.1 | + | 35928 | 0.72 | 0.081688 |
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Target: 5'- gGGagCGGCCAagGCCCGCGACUUGuuCCAGg -3' miRNA: 3'- gCCg-GCCGGU--CGGGCGCUGAGC--GGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 38288 | 1.05 | 0.000177 |
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Target: 5'- uCGGCCGGCCAGCCCGCGACUCGCCAAa -3' miRNA: 3'- -GCCGGCCGGUCGGGCGCUGAGCGGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 38353 | 0.69 | 0.132324 |
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Target: 5'- gGGCUGGCCGGCCgaGCGGaacuaaGCCGu -3' miRNA: 3'- gCCGGCCGGUCGGg-CGCUgag---CGGUu -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 45172 | 0.67 | 0.200188 |
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Target: 5'- cCGGCCcuuGCCAGCUCGa-ACUCGaCCAc -3' miRNA: 3'- -GCCGGc--CGGUCGGGCgcUGAGC-GGUu -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 45733 | 0.67 | 0.190281 |
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Target: 5'- uGGCCGaacuGCCAGUCuCGUGAg-CGCCAGc -3' miRNA: 3'- gCCGGC----CGGUCGG-GCGCUgaGCGGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 46029 | 0.67 | 0.200188 |
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Target: 5'- cCGGCgGGCUucGCCCuucgGUGAUUCGUCAc -3' miRNA: 3'- -GCCGgCCGGu-CGGG----CGCUGAGCGGUu -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 48293 | 0.67 | 0.187394 |
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Target: 5'- gCGGCCGGCCGGCCgaaccacacccuuggCGUGG---GCCAGc -3' miRNA: 3'- -GCCGGCCGGUCGG---------------GCGCUgagCGGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 48359 | 0.76 | 0.036869 |
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Target: 5'- uCGGCCGGCCGG-CCGCGACaagguacggcgcgagCGCCGc -3' miRNA: 3'- -GCCGGCCGGUCgGGCGCUGa--------------GCGGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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