Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1506 | 5' | -65.6 | NC_001335.1 | + | 9662 | 0.68 | 0.166949 |
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Target: 5'- uCGGCCuuGGCCGcguaggugggaguGCUCGgGuCUCGCCAGa -3' miRNA: 3'- -GCCGG--CCGGU-------------CGGGCgCuGAGCGGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 31876 | 0.69 | 0.143185 |
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Target: 5'- cCGGCUGGCUga-CCGgGGCUUGCCGc -3' miRNA: 3'- -GCCGGCCGGucgGGCgCUGAGCGGUu -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 27406 | 0.69 | 0.134788 |
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Target: 5'- aGGCaaucgaugaGGCCacgucgaaggacgaGGCCCGUGACcgCGCCGAg -3' miRNA: 3'- gCCGg--------CCGG--------------UCGGGCGCUGa-GCGGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 38353 | 0.69 | 0.132324 |
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Target: 5'- gGGCUGGCCGGCCgaGCGGaacuaaGCCGu -3' miRNA: 3'- gCCGGCCGGUCGGg-CGCUgag---CGGUu -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 22584 | 0.71 | 0.098701 |
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Target: 5'- gGuGCCGGCCAGCUCGCcGAUaCGCUc- -3' miRNA: 3'- gC-CGGCCGGUCGGGCG-CUGaGCGGuu -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 1876 | 0.71 | 0.093273 |
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Target: 5'- aCGGCCGGggaauacgucCCAGCUCgacaucaGCGAgUCGCCGAu -3' miRNA: 3'- -GCCGGCC----------GGUCGGG-------CGCUgAGCGGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 30095 | 0.71 | 0.088607 |
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Target: 5'- aGGCCGGCgGGUUCGgGcACUCGCuCGAa -3' miRNA: 3'- gCCGGCCGgUCGGGCgC-UGAGCG-GUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 29012 | 0.66 | 0.24439 |
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Target: 5'- gGGCCGGCgGcuGCCgGUGgacaGCUCGCgGAc -3' miRNA: 3'- gCCGGCCGgU--CGGgCGC----UGAGCGgUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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