Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1506 | 5' | -65.6 | NC_001335.1 | + | 48293 | 0.67 | 0.187394 |
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Target: 5'- gCGGCCGGCCGGCCgaaccacacccuuggCGUGG---GCCAGc -3' miRNA: 3'- -GCCGGCCGGUCGG---------------GCGCUgagCGGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 2337 | 0.67 | 0.180808 |
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Target: 5'- gGGCCGGCUcaGGCCgGCGACagGaacuaCCAGa -3' miRNA: 3'- gCCGGCCGG--UCGGgCGCUGagC-----GGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 19786 | 0.67 | 0.176229 |
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Target: 5'- gGGCCGGCCAagugguguuuGCUgGUGACacuguUCGUCAAc -3' miRNA: 3'- gCCGGCCGGU----------CGGgCGCUG-----AGCGGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 31081 | 0.69 | 0.145834 |
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Target: 5'- gGGCCGGUCucaggcuugaccugAGCCaGCGcCUUGCCGAa -3' miRNA: 3'- gCCGGCCGG--------------UCGGgCGCuGAGCGGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 5871 | 0.7 | 0.122227 |
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Target: 5'- aCGGCacaGGCCAGCCCGacccGCUCggGCCu- -3' miRNA: 3'- -GCCGg--CCGGUCGGGCgc--UGAG--CGGuu -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 32157 | 0.73 | 0.073062 |
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Target: 5'- cCGGCCcgcgaacucGGCCAGCUCGuCGGCaucgaagUCGCCGAa -3' miRNA: 3'- -GCCGG---------CCGGUCGGGC-GCUG-------AGCGGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 38288 | 1.05 | 0.000177 |
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Target: 5'- uCGGCCGGCCAGCCCGCGACUCGCCAAa -3' miRNA: 3'- -GCCGGCCGGUCGGGCGCUGAGCGGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 9629 | 0.66 | 0.237862 |
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Target: 5'- aCGGCaaCGGCCAGggugucauCCCGCGugaacguGCUCGCa-- -3' miRNA: 3'- -GCCG--GCCGGUC--------GGGCGC-------UGAGCGguu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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