Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1525 | 5' | -56.6 | NC_001335.1 | + | 37126 | 0.67 | 0.590175 |
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Target: 5'- uGGCGuuGGUgACGGUCUUCacaUCGGcaggaacGUCCa -3' miRNA: 3'- -CCGUggCCG-UGCUAGGAG---AGCC-------UAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 39907 | 0.66 | 0.645145 |
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Target: 5'- uGCGgCGaGUACuGAUCaccggaUCUCGGAUCUg -3' miRNA: 3'- cCGUgGC-CGUG-CUAGg-----AGAGCCUAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 40126 | 0.75 | 0.218036 |
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Target: 5'- gGGCucuCCGGguCGAUCCgCUugUGGGUCCa -3' miRNA: 3'- -CCGu--GGCCguGCUAGGaGA--GCCUAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 41609 | 0.67 | 0.623556 |
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Target: 5'- cGGCcCCaGGCGuCGAUCCcCacgUGGAUCUg -3' miRNA: 3'- -CCGuGG-CCGU-GCUAGGaGa--GCCUAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 41667 | 0.67 | 0.623556 |
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Target: 5'- uGGgGCCGGCugG--UCUCUCGcgacgaGAUCUa -3' miRNA: 3'- -CCgUGGCCGugCuaGGAGAGC------CUAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 42315 | 0.75 | 0.206995 |
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Target: 5'- --gACCGGCACGAuUCCUgCUgCGGAUUCg -3' miRNA: 3'- ccgUGGCCGUGCU-AGGA-GA-GCCUAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 42338 | 0.78 | 0.131357 |
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Target: 5'- cGGCcgACCGGCGCGAgccgcgugCgUCUCGGcGUCCa -3' miRNA: 3'- -CCG--UGGCCGUGCUa-------GgAGAGCC-UAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 48814 | 0.68 | 0.559236 |
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Target: 5'- cGGCgACCGGaCGCGGUagCUUCUCcGGUCa -3' miRNA: 3'- -CCG-UGGCC-GUGCUA--GGAGAGcCUAGg -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 51572 | 1.15 | 0.000333 |
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Target: 5'- cGGCACCGGCACGAUCCUCUCGGAUCCg -3' miRNA: 3'- -CCGUGGCCGUGCUAGGAGAGCCUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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