Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1525 | 5' | -56.6 | NC_001335.1 | + | 2198 | 0.67 | 0.591248 |
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Target: 5'- gGGCGgUGGCGagggucucgaGAUCCUCaacuacgaGGGUCCa -3' miRNA: 3'- -CCGUgGCCGUg---------CUAGGAGag------CCUAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 41609 | 0.67 | 0.623556 |
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Target: 5'- cGGCcCCaGGCGuCGAUCCcCacgUGGAUCUg -3' miRNA: 3'- -CCGuGG-CCGU-GCUAGGaGa--GCCUAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 41667 | 0.67 | 0.623556 |
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Target: 5'- uGGgGCCGGCugG--UCUCUCGcgacgaGAUCUa -3' miRNA: 3'- -CCgUGGCCGugCuaGGAGAGC------CUAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 39907 | 0.66 | 0.645145 |
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Target: 5'- uGCGgCGaGUACuGAUCaccggaUCUCGGAUCUg -3' miRNA: 3'- cCGUgGC-CGUG-CUAGg-----AGAGCCUAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 13095 | 0.66 | 0.665614 |
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Target: 5'- cGCACCGGCACaccggagGAUCagcUUCGuGAUCg -3' miRNA: 3'- cCGUGGCCGUG-------CUAGga-GAGC-CUAGg -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 7506 | 0.66 | 0.666689 |
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Target: 5'- aGCGCCGGC-CGAcaccccggUCUC-CGaGAUCCc -3' miRNA: 3'- cCGUGGCCGuGCUa-------GGAGaGC-CUAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 4113 | 0.66 | 0.666689 |
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Target: 5'- cGGCAgCGGUugGGgaucuugucggCCUCuUCGGAcUCg -3' miRNA: 3'- -CCGUgGCCGugCUa----------GGAG-AGCCUaGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 5544 | 0.66 | 0.666689 |
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Target: 5'- cGGUGCCGaGCAgguaGAUggUCUgUCGGAUCUu -3' miRNA: 3'- -CCGUGGC-CGUg---CUA--GGAgAGCCUAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 32629 | 0.66 | 0.666689 |
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Target: 5'- --gGCCGGCGCgGAUCCaC-CGGAgCCu -3' miRNA: 3'- ccgUGGCCGUG-CUAGGaGaGCCUaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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