Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1525 | 5' | -56.6 | NC_001335.1 | + | 25128 | 0.71 | 0.369279 |
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Target: 5'- uGGCGCUGGCGgcugccuucuucgccCGGUCCUgUggagGGGUCCa -3' miRNA: 3'- -CCGUGGCCGU---------------GCUAGGAgAg---CCUAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 31751 | 0.71 | 0.357468 |
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Target: 5'- aGGCccGCUGGCACGAggCCg--CGGAUgCCg -3' miRNA: 3'- -CCG--UGGCCGUGCUa-GGagaGCCUA-GG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 435 | 0.73 | 0.302458 |
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Target: 5'- cGCACUGGCGCGG-CCUUga-GAUCCg -3' miRNA: 3'- cCGUGGCCGUGCUaGGAGagcCUAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 26696 | 0.73 | 0.295153 |
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Target: 5'- gGGCAUCaGCGCGGUCCUCcgugcuucaUCGGuugucUCCc -3' miRNA: 3'- -CCGUGGcCGUGCUAGGAG---------AGCCu----AGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 16955 | 0.75 | 0.218036 |
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Target: 5'- uGGCgagcacGCCGGCACGAUgCUCacCGGAgcccUCCa -3' miRNA: 3'- -CCG------UGGCCGUGCUAgGAGa-GCCU----AGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 40126 | 0.75 | 0.218036 |
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Target: 5'- gGGCucuCCGGguCGAUCCgCUugUGGGUCCa -3' miRNA: 3'- -CCGu--GGCCguGCUAGGaGA--GCCUAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 42315 | 0.75 | 0.206995 |
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Target: 5'- --gACCGGCACGAuUCCUgCUgCGGAUUCg -3' miRNA: 3'- ccgUGGCCGUGCU-AGGA-GA-GCCUAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 42338 | 0.78 | 0.131357 |
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Target: 5'- cGGCcgACCGGCGCGAgccgcgugCgUCUCGGcGUCCa -3' miRNA: 3'- -CCG--UGGCCGUGCUa-------GgAGAGCC-UAGG- -5' |
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| 1525 | 5' | -56.6 | NC_001335.1 | + | 51572 | 1.15 | 0.000333 |
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Target: 5'- cGGCACCGGCACGAUCCUCUCGGAUCCg -3' miRNA: 3'- -CCGUGGCCGUGCUAGGAGAGCCUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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