Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16250 | 5' | -52 | NC_004084.1 | + | 51186 | 0.67 | 0.870736 |
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Target: 5'- gCGCCgaucGUCG-AGGUCGGCgcuggcgCGGGCUa -3' miRNA: 3'- -GCGGaa--CAGCgUCUAGCUGa------GCUUGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 52286 | 0.66 | 0.913809 |
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Target: 5'- gGCgCUgGUCGCGGAaucgcaUCGACcUGAACg -3' miRNA: 3'- gCG-GAaCAGCGUCU------AGCUGaGCUUGg -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 53187 | 0.66 | 0.886158 |
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Target: 5'- aGCCaacUUGUCGagucuuccguGAUCGuCUCGAGCg -3' miRNA: 3'- gCGG---AACAGCgu--------CUAGCuGAGCUUGg -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 53233 | 0.75 | 0.44844 |
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Target: 5'- cCGCCUcgucuacccaGUCGUcGAUCGACUCGAgugcuGCCu -3' miRNA: 3'- -GCGGAa---------CAGCGuCUAGCUGAGCU-----UGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 53406 | 0.7 | 0.684727 |
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Target: 5'- aGCCcgUUGauccccUCuCGGAUCGACUCGAGCg -3' miRNA: 3'- gCGG--AAC------AGcGUCUAGCUGAGCUUGg -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 55209 | 0.68 | 0.827928 |
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Target: 5'- gGCCgacacgGUacaccCGCAGAUCGcuCUCgGAACCg -3' miRNA: 3'- gCGGaa----CA-----GCGUCUAGCu-GAG-CUUGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 56831 | 0.67 | 0.854312 |
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Target: 5'- uGCCaaccagCGCGGGccUCGAgcCUCGGGCCa -3' miRNA: 3'- gCGGaaca--GCGUCU--AGCU--GAGCUUGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 57630 | 0.69 | 0.759559 |
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Target: 5'- uCGUCUcgagGUCGCGcGUCGcCUCGAcgaGCCg -3' miRNA: 3'- -GCGGAa---CAGCGUcUAGCuGAGCU---UGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 57871 | 1.04 | 0.006091 |
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Target: 5'- cCGCCUUGUCGCAG-UCGACUCGAACCg -3' miRNA: 3'- -GCGGAACAGCGUCuAGCUGAGCUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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