Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16250 | 5' | -52 | NC_004084.1 | + | 53406 | 0.7 | 0.684727 |
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Target: 5'- aGCCcgUUGauccccUCuCGGAUCGACUCGAGCg -3' miRNA: 3'- gCGG--AAC------AGcGUCUAGCUGAGCUUGg -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 397 | 0.71 | 0.662716 |
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Target: 5'- cCGCCgaUGUCGCAGAcgucgUCGAUcUGGACg -3' miRNA: 3'- -GCGGa-ACAGCGUCU-----AGCUGaGCUUGg -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 38874 | 0.72 | 0.622859 |
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Target: 5'- aGCCagaGUUGCGGGaagaagacagcgucuUCGAgCUCGAACCg -3' miRNA: 3'- gCGGaa-CAGCGUCU---------------AGCU-GAGCUUGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 10244 | 0.73 | 0.541729 |
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Target: 5'- uCGCCUggugaGUCGCGG-UCGGCgucCGGAUCa -3' miRNA: 3'- -GCGGAa----CAGCGUCuAGCUGa--GCUUGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 18575 | 0.73 | 0.530994 |
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Target: 5'- gCGCCgcgGcCGCcGAUgGACUCGAGCg -3' miRNA: 3'- -GCGGaa-CaGCGuCUAgCUGAGCUUGg -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 4191 | 0.73 | 0.520338 |
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Target: 5'- uCGCUccgacGUCG-AGAUcCGACUCGAGCCg -3' miRNA: 3'- -GCGGaa---CAGCgUCUA-GCUGAGCUUGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 47137 | 0.74 | 0.488902 |
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Target: 5'- uCGCCcucacUGUU-CAGGUCGAgCUCGAACCg -3' miRNA: 3'- -GCGGa----ACAGcGUCUAGCU-GAGCUUGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 53233 | 0.75 | 0.44844 |
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Target: 5'- cCGCCUcgucuacccaGUCGUcGAUCGACUCGAgugcuGCCu -3' miRNA: 3'- -GCGGAa---------CAGCGuCUAGCUGAGCU-----UGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 57871 | 1.04 | 0.006091 |
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Target: 5'- cCGCCUUGUCGCAG-UCGACUCGAACCg -3' miRNA: 3'- -GCGGAACAGCGUCuAGCUGAGCUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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