miRNA: 3'- aGCAGUAGUAG-GAG-----------GAGCgCUAGGUC- -5'
miRNA: 3'- -AGCAGuaGUAGgAGgaGC-GCU--AGGUC- -5'
miRNA: 3'- aGCAGUa--GUAGGA------GGAGCGCU---AGGUC- -5'
miRNA: 3'- -AGCAGUAGUAGGAGGAGCGCUAGGUC- -5'
miRNA: 3'- -AGCAGUAGUAGGAGgAGC-GCuAGguC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16254 | 5' | -53.5 | NC_004084.1 | + | 45879 | 0.71 | 0.551524 |
|
Target: 5'- aCGUCGUCGUCgCUCaccugcaagcuCUCG-GAUCCAGc -3' miRNA: 3'- aGCAGUAGUAG-GAG-----------GAGCgCUAGGUC- -5' |
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| 16254 | 5' | -53.5 | NC_004084.1 | + | 47377 | 0.66 | 0.826223 |
|
Target: 5'- uUCGUCcgCGUCgUCgaCGgCGAgcUCCAGg -3' miRNA: 3'- -AGCAGuaGUAGgAGgaGC-GCU--AGGUC- -5' |
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| 16254 | 5' | -53.5 | NC_004084.1 | + | 47954 | 0.74 | 0.409496 |
|
Target: 5'- cCGUCAggcCGUCCUggaucgCCUCGCGGgcuUCCGGg -3' miRNA: 3'- aGCAGUa--GUAGGA------GGAGCGCU---AGGUC- -5' |
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| 16254 | 5' | -53.5 | NC_004084.1 | + | 55941 | 1.09 | 0.001865 |
|
Target: 5'- gUCGUCAUCAUCCUCCUCGCGAUCCAGc -3' miRNA: 3'- -AGCAGUAGUAGGAGGAGCGCUAGGUC- -5' |
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| 16254 | 5' | -53.5 | NC_004084.1 | + | 56102 | 0.73 | 0.418898 |
|
Target: 5'- cUCGUCAUCGUCCUCgUCGaCG-UCguGc -3' miRNA: 3'- -AGCAGUAGUAGGAGgAGC-GCuAGguC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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