Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16254 | 5' | -53.5 | NC_004084.1 | + | 13137 | 0.69 | 0.643652 |
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Target: 5'- cCGUCGUCGUacucgaCCUCCUucggguacacacagaUGCGGUcCCAGg -3' miRNA: 3'- aGCAGUAGUA------GGAGGA---------------GCGCUA-GGUC- -5' |
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| 16254 | 5' | -53.5 | NC_004084.1 | + | 11399 | 0.69 | 0.639237 |
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Target: 5'- aUCGUCAUCgaucgcgacGUCCUCgaggaaCUCGCGGUCg-- -3' miRNA: 3'- -AGCAGUAG---------UAGGAG------GAGCGCUAGguc -5' |
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| 16254 | 5' | -53.5 | NC_004084.1 | + | 9633 | 0.75 | 0.331072 |
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Target: 5'- cUCGUUgcgcggAUCGUCCUCCUCGCGcuUCCc- -3' miRNA: 3'- -AGCAG------UAGUAGGAGGAGCGCu-AGGuc -5' |
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| 16254 | 5' | -53.5 | NC_004084.1 | + | 4355 | 0.67 | 0.757891 |
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Target: 5'- cUCGccCGUC-UCCUCgUCGCGcGUCCAa -3' miRNA: 3'- -AGCa-GUAGuAGGAGgAGCGC-UAGGUc -5' |
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| 16254 | 5' | -53.5 | NC_004084.1 | + | 3055 | 0.84 | 0.090806 |
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Target: 5'- aUCGUCGUCAUCCUCCUugcuguuuggCGCGAUCUc- -3' miRNA: 3'- -AGCAGUAGUAGGAGGA----------GCGCUAGGuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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