Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16267 | 5' | -54.5 | NC_004084.1 | + | 15720 | 0.66 | 0.828283 |
Target: 5'- aGGGuCGucaGCAUCGGu-GAUCGcUGGCa -3' miRNA: 3'- gCCCuGC---CGUAGCCuuCUAGC-ACUGg -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 29027 | 0.66 | 0.820179 |
Target: 5'- uCGaGGGCGGCAUCaccuuccaGGAGGGacgcgggaucccgggCGUcGACCa -3' miRNA: 3'- -GC-CCUGCCGUAG--------CCUUCUa--------------GCA-CUGG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 56616 | 0.66 | 0.810065 |
Target: 5'- cCGGu-CGGCGaguUCGccGGAUCGUGGCUc -3' miRNA: 3'- -GCCcuGCCGU---AGCcuUCUAGCACUGG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 55962 | 0.66 | 0.80068 |
Target: 5'- cCGGGuacgACGGCGacgCGGAGGggC-UGAUCg -3' miRNA: 3'- -GCCC----UGCCGUa--GCCUUCuaGcACUGG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 29583 | 0.66 | 0.797831 |
Target: 5'- cCGGGugGGCGUCGuucgcgcgcaguucGAUgGUGAgCg -3' miRNA: 3'- -GCCCugCCGUAGCcuu-----------CUAgCACUgG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 49079 | 0.66 | 0.791124 |
Target: 5'- aCGuGcGACGGU--CGGGAGAggucucgCGUGACUg -3' miRNA: 3'- -GC-C-CUGCCGuaGCCUUCUa------GCACUGG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 53276 | 0.66 | 0.781407 |
Target: 5'- aGGGACGaCGUCGGucgccacgccGAGGUUGUaucgacggucGGCCa -3' miRNA: 3'- gCCCUGCcGUAGCC----------UUCUAGCA----------CUGG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 7548 | 0.66 | 0.781407 |
Target: 5'- -aGGACGGCAUCGaGAAGAaC---GCCg -3' miRNA: 3'- gcCCUGCCGUAGC-CUUCUaGcacUGG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 45676 | 0.67 | 0.77154 |
Target: 5'- aCGGu-CGGCGUCGacGAGAUCGUGcGCg -3' miRNA: 3'- -GCCcuGCCGUAGCc-UUCUAGCAC-UGg -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 21767 | 0.67 | 0.77154 |
Target: 5'- aCGGGgaucgGCGGCAUCGac--GUCGgGGCCg -3' miRNA: 3'- -GCCC-----UGCCGUAGCcuucUAGCaCUGG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 37866 | 0.67 | 0.77154 |
Target: 5'- aCGGGAUGGCcgggCGGGu-GUCG-GAUCg -3' miRNA: 3'- -GCCCUGCCGua--GCCUucUAGCaCUGG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 53247 | 0.67 | 0.761534 |
Target: 5'- gCGGG-CGGuCGUCGacggcgacGAGAUCG-GGCCg -3' miRNA: 3'- -GCCCuGCC-GUAGCc-------UUCUAGCaCUGG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 13477 | 0.67 | 0.749357 |
Target: 5'- uCGGGcuaaccaacgaGGUGUCGGAAGccAUCGgacugGACCg -3' miRNA: 3'- -GCCCug---------CCGUAGCCUUC--UAGCa----CUGG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 39256 | 0.67 | 0.741146 |
Target: 5'- -aGGGCGGCG--GGAAGAUgGUGGUCg -3' miRNA: 3'- gcCCUGCCGUagCCUUCUAgCACUGG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 46572 | 0.67 | 0.741146 |
Target: 5'- uCGGcauCGGCAUCGcGgcGAUCGUcGugCu -3' miRNA: 3'- -GCCcu-GCCGUAGC-CuuCUAGCA-CugG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 9853 | 0.67 | 0.741146 |
Target: 5'- gCGaGGGCGGCucacgCGGGAGcccggcggaagaGUCGgcGACCg -3' miRNA: 3'- -GC-CCUGCCGua---GCCUUC------------UAGCa-CUGG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 16701 | 0.68 | 0.699176 |
Target: 5'- cCGGGACG--AUCuGAAGGUCGaaGACCg -3' miRNA: 3'- -GCCCUGCcgUAGcCUUCUAGCa-CUGG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 16927 | 0.68 | 0.688498 |
Target: 5'- cCGGGugcgguuCGGCAUCGGcGGGcgUCGcGACg -3' miRNA: 3'- -GCCCu------GCCGUAGCCuUCU--AGCaCUGg -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 44823 | 0.69 | 0.656193 |
Target: 5'- uCGGuGACGGUAUCGaGcugaccGAGGUCG-GAUCg -3' miRNA: 3'- -GCC-CUGCCGUAGC-C------UUCUAGCaCUGG- -5' |
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16267 | 5' | -54.5 | NC_004084.1 | + | 33006 | 0.69 | 0.651865 |
Target: 5'- uCGGGAUGcGgAUCgGGGAGAUCGcccacaugaagcgGACCu -3' miRNA: 3'- -GCCCUGC-CgUAG-CCUUCUAGCa------------CUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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