miRNA: 3'- -GC-CCUGCCGUAGcCUUCUA-GCacUGG- -5'
miRNA: 3'- gCC-CUGCCGuAGCCUucUAGCACUGG- -5'
miRNA: 3'- gCCCUGCCGUAGCcu--UCUA---GC--ACUGG- -5'
miRNA: 3'- -GCCCugCCGuag--CCUUCUAGCaCUGg -5'
miRNA: 3'- -GCCCUGCCGUAGCCUUCUAGCACUGG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16267 | 5' | -54.5 | NC_004084.1 | + | 25475 | 0.69 | 0.645368 |
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Target: 5'- uCGcGGGCGGCGUCaGAAGGUgCGc-GCCg -3' miRNA: 3'- -GC-CCUGCCGUAGcCUUCUA-GCacUGG- -5' |
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| 16267 | 5' | -54.5 | NC_004084.1 | + | 19401 | 0.7 | 0.591255 |
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Target: 5'- aGGcGAUGGUuUCGGA--GUCGUGAUCc -3' miRNA: 3'- gCC-CUGCCGuAGCCUucUAGCACUGG- -5' |
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| 16267 | 5' | -54.5 | NC_004084.1 | + | 12715 | 0.71 | 0.517087 |
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Target: 5'- uGGGGCGGCAUCGaucuGGAUgccCGacUGGCCc -3' miRNA: 3'- gCCCUGCCGUAGCcu--UCUA---GC--ACUGG- -5' |
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| 16267 | 5' | -54.5 | NC_004084.1 | + | 18556 | 0.73 | 0.400177 |
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Target: 5'- uCGGGugGGCcgacgGGGAGAUCGcGACg -3' miRNA: 3'- -GCCCugCCGuag--CCUUCUAGCaCUGg -5' |
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| 16267 | 5' | -54.5 | NC_004084.1 | + | 49048 | 1.13 | 0.000805 |
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Target: 5'- aCGGGACGGCAUCGGAAGAUCGUGACCg -3' miRNA: 3'- -GCCCUGCCGUAGCCUUCUAGCACUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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