Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16566 | 5' | -49.7 | NC_004117.1 | + | 131409 | 0.67 | 0.993731 |
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Target: 5'- uUGUGCAGCACacauCUGuCGAGCGGAu -3' miRNA: 3'- -ACGCGUUGUGcau-GGCuGUUCGUUUu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 133513 | 0.67 | 0.991618 |
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Target: 5'- cGCGacaGAUACGauaugGgCGACAGGCAGAu -3' miRNA: 3'- aCGCg--UUGUGCa----UgGCUGUUCGUUUu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 135661 | 0.67 | 0.988978 |
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Target: 5'- gGCGUAcgccccugGCAucuauUGUGCCGACuuuAGCAAGAu -3' miRNA: 3'- aCGCGU--------UGU-----GCAUGGCUGu--UCGUUUU- -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 29734 | 0.68 | 0.983856 |
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Target: 5'- uUGUuuuCAACAcCGUaACCGACGAGCAAc- -3' miRNA: 3'- -ACGc--GUUGU-GCA-UGGCUGUUCGUUuu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 141225 | 0.68 | 0.981799 |
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Target: 5'- cGCGCGACACGUuUCG-CA-GCAGu- -3' miRNA: 3'- aCGCGUUGUGCAuGGCuGUuCGUUuu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 8294 | 0.68 | 0.979552 |
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Target: 5'- cGCGCGuguacgauACACGUGCCGAUuacguGCu--- -3' miRNA: 3'- aCGCGU--------UGUGCAUGGCUGuu---CGuuuu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 71463 | 0.69 | 0.977106 |
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Target: 5'- aUGCGCAugGCGuUGCCGGCc-GUAc-- -3' miRNA: 3'- -ACGCGUugUGC-AUGGCUGuuCGUuuu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 42150 | 0.69 | 0.97445 |
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Target: 5'- uUGCGCGACAC--AUCGACAuuggugccuAGCAAu- -3' miRNA: 3'- -ACGCGUUGUGcaUGGCUGU---------UCGUUuu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 96896 | 0.7 | 0.957761 |
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Target: 5'- cGCGuCAaagcACACGcUACCGAucCGAGCAAAu -3' miRNA: 3'- aCGC-GU----UGUGC-AUGGCU--GUUCGUUUu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 43218 | 0.66 | 0.995394 |
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Target: 5'- cGUGCGGCugGcauCCGACAGGa---- -3' miRNA: 3'- aCGCGUUGugCau-GGCUGUUCguuuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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