Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16566 | 5' | -49.7 | NC_004117.1 | + | 51122 | 0.71 | 0.929438 |
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Target: 5'- uUGUGCAACGCGaACagGAgGAGCGGGAa -3' miRNA: 3'- -ACGCGUUGUGCaUGg-CUgUUCGUUUU- -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 63647 | 0.71 | 0.929438 |
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Target: 5'- cGCGCGGCA-GUugaucGCCGACAAGUg--- -3' miRNA: 3'- aCGCGUUGUgCA-----UGGCUGUUCGuuuu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 151932 | 0.71 | 0.923787 |
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Target: 5'- aGUGUGGCACGUAUuCGGCGuguGCAAGAg -3' miRNA: 3'- aCGCGUUGUGCAUG-GCUGUu--CGUUUU- -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 118411 | 0.71 | 0.91604 |
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Target: 5'- cGCGCGAucgauucugccaguCGCGUGCCGugGcAGCAu-- -3' miRNA: 3'- aCGCGUU--------------GUGCAUGGCugU-UCGUuuu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 80344 | 0.72 | 0.882086 |
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Target: 5'- uUGCGCAaauaccauaacaccACACGUGgCGACuugGGCAAAc -3' miRNA: 3'- -ACGCGU--------------UGUGCAUgGCUGu--UCGUUUu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 104433 | 0.73 | 0.876822 |
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Target: 5'- -uCGCAGagaGCGUACCGACAGGuCGAu- -3' miRNA: 3'- acGCGUUg--UGCAUGGCUGUUC-GUUuu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 142093 | 0.73 | 0.861126 |
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Target: 5'- -aCGCuucuGACA-GUGCCGACGAGCAAAAc -3' miRNA: 3'- acGCG----UUGUgCAUGGCUGUUCGUUUU- -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 41986 | 0.73 | 0.844502 |
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Target: 5'- cGCGCAACACGUuucgcaGCCacuggGACGAGCu--- -3' miRNA: 3'- aCGCGUUGUGCA------UGG-----CUGUUCGuuuu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 78414 | 0.76 | 0.697555 |
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Target: 5'- uUGgGCAACGCGU-CCGGCA-GCAAAc -3' miRNA: 3'- -ACgCGUUGUGCAuGGCUGUuCGUUUu -5' |
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| 16566 | 5' | -49.7 | NC_004117.1 | + | 19235 | 1.07 | 0.013832 |
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Target: 5'- uUGCGCAACACGUACCGACAAGCAAAAu -3' miRNA: 3'- -ACGCGUUGUGCAUGGCUGUUCGUUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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