Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16626 | 5' | -48.3 | NC_004156.1 | + | 186220 | 0.66 | 0.999904 |
Target: 5'- aGCAUCUCCAUuGCuAgCAACGAUgCUg -3' miRNA: 3'- aCGUAGGGGUAuUG-UgGUUGUUA-GAg -5' |
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16626 | 5' | -48.3 | NC_004156.1 | + | 190551 | 0.66 | 0.999793 |
Target: 5'- aUGCAUCU----GACGCCGuugcuCAAUCUCa -3' miRNA: 3'- -ACGUAGGgguaUUGUGGUu----GUUAGAG- -5' |
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16626 | 5' | -48.3 | NC_004156.1 | + | 199383 | 0.72 | 0.981585 |
Target: 5'- -cCGUCUgCGUGAUGCCGACAuugGUCUCg -3' miRNA: 3'- acGUAGGgGUAUUGUGGUUGU---UAGAG- -5' |
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16626 | 5' | -48.3 | NC_004156.1 | + | 207210 | 0.68 | 0.998222 |
Target: 5'- cGUAUCCUCuacUGGCGCCGuaccgagugcccaGCAAUCUg -3' miRNA: 3'- aCGUAGGGGu--AUUGUGGU-------------UGUUAGAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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