miRNA: 3'- aCGUaGGGGUaUUGUGGUUGUuAGAg -5'
miRNA: 3'- aCGUAGGGGUaUUG-UGGUUGU--UAGAg -5'
miRNA: 3'- -ACGUAGGGGUAUUGUGGUUGUUAGAG- -5'
miRNA: 3'- aCGUaGGGGUAuuGUGGUUGUUAgAG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16626 | 5' | -48.3 | NC_004156.1 | + | 44452 | 0.66 | 0.999875 |
|
Target: 5'- aGCA-CCUCA-AGCACCGGCA-UCUg -3' miRNA: 3'- aCGUaGGGGUaUUGUGGUUGUuAGAg -5' |
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| 16626 | 5' | -48.3 | NC_004156.1 | + | 12560 | 0.72 | 0.981585 |
|
Target: 5'- cGCAUCCUCA-AACuGCCAACAgcAUCUa -3' miRNA: 3'- aCGUAGGGGUaUUG-UGGUUGU--UAGAg -5' |
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| 16626 | 5' | -48.3 | NC_004156.1 | + | 8228 | 1.12 | 0.013953 |
|
Target: 5'- gUGCAUCCCCAUAACACCAACAAUCUCa -3' miRNA: 3'- -ACGUAGGGGUAUUGUGGUUGUUAGAG- -5' |
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| 16626 | 5' | -48.3 | NC_004156.1 | + | 8198 | 0.73 | 0.965166 |
|
Target: 5'- aGCAaCCCCAU--CAUCAGCAGUgUCa -3' miRNA: 3'- aCGUaGGGGUAuuGUGGUUGUUAgAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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