Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16633 | 5' | -44.6 | NC_004156.1 | + | 184591 | 0.66 | 1 |
Target: 5'- gAC-CACGGUC----AUcGAGCAGGCg -3' miRNA: 3'- aUGuGUGCCAGauuuUGaCUUGUUCG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 29051 | 0.67 | 1 |
Target: 5'- aUugGCAU-GUUUGAGACUG-ACGAGUa -3' miRNA: 3'- -AugUGUGcCAGAUUUUGACuUGUUCG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 39188 | 0.66 | 1 |
Target: 5'- aUACAgGCGGUggugaaucgauucCUGGGuGCUGuGCAAGCc -3' miRNA: 3'- -AUGUgUGCCA-------------GAUUU-UGACuUGUUCG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 69420 | 0.66 | 1 |
Target: 5'- aGCuuugaACGGUCUGcguGugUGGGCAAGa -3' miRNA: 3'- aUGug---UGCCAGAUu--UugACUUGUUCg -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 133828 | 0.66 | 1 |
Target: 5'- ---gUACGGUCUGAGACUGuu---GCc -3' miRNA: 3'- auguGUGCCAGAUUUUGACuuguuCG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 110472 | 0.66 | 1 |
Target: 5'- gUACugGaCGGUCUGcacGCUGAuCAGGa -3' miRNA: 3'- -AUGugU-GCCAGAUuu-UGACUuGUUCg -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 85301 | 0.66 | 1 |
Target: 5'- cGCACcuCGGUCUAucACaaaaUGAACAAuauGCa -3' miRNA: 3'- aUGUGu-GCCAGAUuuUG----ACUUGUU---CG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 70415 | 0.66 | 1 |
Target: 5'- aACGCuAaaGUCUAAA-CUGAGCGAGg -3' miRNA: 3'- aUGUG-UgcCAGAUUUuGACUUGUUCg -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 14520 | 0.66 | 1 |
Target: 5'- --uGCAUGGUCcaguuGACUuuGCGAGCa -3' miRNA: 3'- augUGUGCCAGauu--UUGAcuUGUUCG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 49126 | 0.66 | 1 |
Target: 5'- gACACACGGuuguugauucugauUCUGAuuCUGAGucUGAGUa -3' miRNA: 3'- aUGUGUGCC--------------AGAUUuuGACUU--GUUCG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 170046 | 0.67 | 0.999999 |
Target: 5'- gACaACACGaGUCUGguAAGCUuuguACAAGCg -3' miRNA: 3'- aUG-UGUGC-CAGAU--UUUGAcu--UGUUCG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 213373 | 0.67 | 0.999999 |
Target: 5'- --uGCACGGUC-GAGACUauuacaaGAGCGAuGCa -3' miRNA: 3'- augUGUGCCAGaUUUUGA-------CUUGUU-CG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 33623 | 0.67 | 0.999999 |
Target: 5'- uUACACGaguccacCGaGUCUAAGAUUGcACGAGUc -3' miRNA: 3'- -AUGUGU-------GC-CAGAUUUUGACuUGUUCG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 158789 | 0.67 | 0.999999 |
Target: 5'- gUGCACACuGGaUCcGAAACUGGuacuuuuGCAGGUu -3' miRNA: 3'- -AUGUGUG-CC-AGaUUUUGACU-------UGUUCG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 14501 | 0.67 | 0.999998 |
Target: 5'- uUAUACAUGGUauuuaUUAAAACU-AGCAAGUa -3' miRNA: 3'- -AUGUGUGCCA-----GAUUUUGAcUUGUUCG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 19700 | 0.67 | 0.999998 |
Target: 5'- aGCAC-CGGUCgauuggucuuGAGCUGA--GAGCg -3' miRNA: 3'- aUGUGuGCCAGau--------UUUGACUugUUCG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 157815 | 0.68 | 0.999997 |
Target: 5'- aACACAUa-UUUGGAAgUGAACGAGCc -3' miRNA: 3'- aUGUGUGccAGAUUUUgACUUGUUCG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 186193 | 0.68 | 0.999992 |
Target: 5'- gGCACugGcaCUGGuACUGGACuAGCa -3' miRNA: 3'- aUGUGugCcaGAUUuUGACUUGuUCG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 180431 | 0.68 | 0.999992 |
Target: 5'- cACAgACGGaUCgcAAACUGGACAuuuccguGCa -3' miRNA: 3'- aUGUgUGCC-AGauUUUGACUUGUu------CG- -5' |
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16633 | 5' | -44.6 | NC_004156.1 | + | 42534 | 0.69 | 0.999989 |
Target: 5'- cUGgGCACGGUCgguguccGugUGGGCAAGa -3' miRNA: 3'- -AUgUGUGCCAGauu----UugACUUGUUCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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