Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16634 | 3' | -54.1 | NC_004156.1 | + | 59105 | 0.66 | 0.956763 |
Target: 5'- gGACGaagAGAGUcguaagGAGGCUGcuGGUAGCAc -3' miRNA: 3'- -UUGCa--UUUCGa-----CUCCGACu-CCGUCGU- -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 57996 | 0.67 | 0.934626 |
Target: 5'- aAACGcAAAGCgGAGGCUGAaGCuGCc -3' miRNA: 3'- -UUGCaUUUCGaCUCCGACUcCGuCGu -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 58026 | 0.67 | 0.934626 |
Target: 5'- aAACGcAAAGCgGAGGCUGAaGCuGCc -3' miRNA: 3'- -UUGCaUUUCGaCUCCGACUcCGuCGu -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 57735 | 0.68 | 0.912527 |
Target: 5'- aAACGaAAAGCgGAGGCUGAaGCuGCu -3' miRNA: 3'- -UUGCaUUUCGaCUCCGACUcCGuCGu -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 57825 | 0.68 | 0.912527 |
Target: 5'- aAACGcAAAGCgGAGGCUGAaGCuGCu -3' miRNA: 3'- -UUGCaUUUCGaCUCCGACUcCGuCGu -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 58269 | 0.68 | 0.912527 |
Target: 5'- aAGCGcAAAGCgGAGGCUGAaGCuGCu -3' miRNA: 3'- -UUGCaUUUCGaCUCCGACUcCGuCGu -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 58544 | 0.7 | 0.856865 |
Target: 5'- aAGCGaAAGGCUGAuGCUGAaGCAGCc -3' miRNA: 3'- -UUGCaUUUCGACUcCGACUcCGUCGu -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 58511 | 0.7 | 0.823851 |
Target: 5'- aAACGUAAAGCcGAuGCUGAaGCAGCc -3' miRNA: 3'- -UUGCAUUUCGaCUcCGACUcCGUCGu -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 85640 | 0.72 | 0.72 |
Target: 5'- aGACGUAAAGCggGAGGCuccuuUGAagcGGUAGCGu -3' miRNA: 3'- -UUGCAUUUCGa-CUCCG-----ACU---CCGUCGU- -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 58086 | 0.73 | 0.658585 |
Target: 5'- aAACGUAAAGCUGAGaUUGAaGCAGCc -3' miRNA: 3'- -UUGCAUUUCGACUCcGACUcCGUCGu -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 58625 | 0.74 | 0.617078 |
Target: 5'- aAACGUAAAGCcGAuGCUGAaGCAGCAa -3' miRNA: 3'- -UUGCAUUUCGaCUcCGACUcCGUCGU- -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 58571 | 0.74 | 0.617078 |
Target: 5'- aAACGUAAAGCcGAuGCUGAaGCAGCAa -3' miRNA: 3'- -UUGCAUUUCGaCUcCGACUcCGUCGU- -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 58478 | 0.74 | 0.617078 |
Target: 5'- aAACGUAAAGCcGAuGCUGAaGCAGCAa -3' miRNA: 3'- -UUGCAUUUCGaCUcCGACUcCGUCGU- -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 58389 | 0.75 | 0.596362 |
Target: 5'- aAACGcAAAGCgGAGGCUGAaGCAGCc -3' miRNA: 3'- -UUGCaUUUCGaCUCCGACUcCGUCGu -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 58119 | 0.75 | 0.596362 |
Target: 5'- aAACGcAAAGCgGAGGCUGAaGCAGCc -3' miRNA: 3'- -UUGCaUUUCGaCUCCGACUcCGUCGu -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 57672 | 0.75 | 0.565509 |
Target: 5'- aAACGUAAAGCcGAGGCUGAaGCuGCc -3' miRNA: 3'- -UUGCAUUUCGaCUCCGACUcCGuCGu -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 58056 | 0.76 | 0.525069 |
Target: 5'- aAACGUAAAGCgGAGGCUGAaGCuGCc -3' miRNA: 3'- -UUGCAUUUCGaCUCCGACUcCGuCGu -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 224714 | 0.78 | 0.429635 |
Target: 5'- uGAgGuUGAGGUUGAGGUUGAGGCAGUu -3' miRNA: 3'- -UUgC-AUUUCGACUCCGACUCCGUCGu -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 58179 | 0.85 | 0.170352 |
Target: 5'- aAACGUAAAGCcGAGGCUGAaGCGGCAg -3' miRNA: 3'- -UUGCAUUUCGaCUCCGACUcCGUCGU- -5' |
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16634 | 3' | -54.1 | NC_004156.1 | + | 58239 | 0.85 | 0.170352 |
Target: 5'- aAACGUAAAGCcGAGGCUGAaGCGGCAg -3' miRNA: 3'- -UUGCAUUUCGaCUCCGACUcCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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