Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16636 | 3' | -47.3 | NC_004156.1 | + | 91999 | 0.66 | 0.999979 |
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Target: 5'- cAAGC-GUAggcGUCAGGUUGACGAUg- -3' miRNA: 3'- uUUCGaUAUa--CAGUUCAGCUGCUGgg -5' |
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| 16636 | 3' | -47.3 | NC_004156.1 | + | 184846 | 0.67 | 0.99992 |
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Target: 5'- -uGGCUA-AUGUCGAGUCGGCcaaacuuugaaauuGCCUa -3' miRNA: 3'- uuUCGAUaUACAGUUCAGCUGc-------------UGGG- -5' |
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| 16636 | 3' | -47.3 | NC_004156.1 | + | 75372 | 0.68 | 0.999807 |
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Target: 5'- gAAGGCgAUAuUGcCAAGUUGACGGCUUu -3' miRNA: 3'- -UUUCGaUAU-ACaGUUCAGCUGCUGGG- -5' |
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| 16636 | 3' | -47.3 | NC_004156.1 | + | 75602 | 0.69 | 0.999074 |
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Target: 5'- -cAGuCUGUGUGUCAGG-CuGCGGCCa -3' miRNA: 3'- uuUC-GAUAUACAGUUCaGcUGCUGGg -5' |
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| 16636 | 3' | -47.3 | NC_004156.1 | + | 9727 | 0.69 | 0.998628 |
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Target: 5'- cAAAGUgAUGUGgaUCAGGUCGACGAaCUg -3' miRNA: 3'- -UUUCGaUAUAC--AGUUCAGCUGCUgGG- -5' |
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| 16636 | 3' | -47.3 | NC_004156.1 | + | 62913 | 1.12 | 0.019468 |
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Target: 5'- cAAAGCUAUAUGUCAAGUCGACGACCCg -3' miRNA: 3'- -UUUCGAUAUACAGUUCAGCUGCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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