Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16637 | 3' | -45.6 | NC_004156.1 | + | 136155 | 0.66 | 0.999999 |
Target: 5'- gGCCGCCgAGGGAaugggauagagUGCAUugUCGAUa -3' miRNA: 3'- gUGGCGGgUUUCUaaa--------ACGUA--AGUUG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 75760 | 0.66 | 0.999999 |
Target: 5'- aGCUGCgCGAguuGGAUcggUGCAUUCAGg -3' miRNA: 3'- gUGGCGgGUU---UCUAaa-ACGUAAGUUg -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 73353 | 0.66 | 0.999999 |
Target: 5'- aGCCGUCCucAGuAUgguacucGCGUUCGACa -3' miRNA: 3'- gUGGCGGGuuUC-UAaaa----CGUAAGUUG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 165882 | 0.66 | 0.999999 |
Target: 5'- gACgCGCUCGAAGugUUUGguUUCGAUg -3' miRNA: 3'- gUG-GCGGGUUUCuaAAACguAAGUUG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 18720 | 0.66 | 0.999999 |
Target: 5'- cUACCGCCCAuAGAUg-UGaCcgUgAGCg -3' miRNA: 3'- -GUGGCGGGUuUCUAaaAC-GuaAgUUG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 210189 | 0.66 | 0.999999 |
Target: 5'- gCACUGCCCGAucuggcAGAggccGCAgccgCAGCa -3' miRNA: 3'- -GUGGCGGGUU------UCUaaaaCGUaa--GUUG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 175145 | 0.66 | 0.999998 |
Target: 5'- -gUCGUCCAGAGAg--UGCAUcuUCAuGCa -3' miRNA: 3'- guGGCGGGUUUCUaaaACGUA--AGU-UG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 31882 | 0.66 | 0.999998 |
Target: 5'- aGCCGCCCu--GGU--UGCAgagugCAACc -3' miRNA: 3'- gUGGCGGGuuuCUAaaACGUaa---GUUG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 81186 | 0.66 | 0.999998 |
Target: 5'- aCGCCGgCCAcugcagugacuaGGGAcacgUUUGgAUUCAGCg -3' miRNA: 3'- -GUGGCgGGU------------UUCUa---AAACgUAAGUUG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 186518 | 0.66 | 0.999997 |
Target: 5'- -uCUGCCCAuAGAUUUgaaGCAcUUUGACa -3' miRNA: 3'- guGGCGGGUuUCUAAAa--CGU-AAGUUG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 180834 | 0.67 | 0.999995 |
Target: 5'- --gCGUCCAAAGugaUUGCAUgCAACa -3' miRNA: 3'- gugGCGGGUUUCuaaAACGUAaGUUG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 127495 | 0.67 | 0.999995 |
Target: 5'- aCGCUgauGCCUcagauuauAAAGAUUUUGCucUUCAACu -3' miRNA: 3'- -GUGG---CGGG--------UUUCUAAAACGu-AAGUUG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 169888 | 0.67 | 0.999993 |
Target: 5'- cCACUGCCCGgcuuGAGcAUcUUGCcgUCGAg -3' miRNA: 3'- -GUGGCGGGU----UUC-UAaAACGuaAGUUg -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 207161 | 0.67 | 0.999993 |
Target: 5'- cUACCGCCaaggguGGAGGUUUUGaauacUUCGGCa -3' miRNA: 3'- -GUGGCGGg-----UUUCUAAAACgu---AAGUUG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 102177 | 0.67 | 0.999993 |
Target: 5'- aCACUGCCUuuAGAUg--GCA--CAACa -3' miRNA: 3'- -GUGGCGGGuuUCUAaaaCGUaaGUUG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 159485 | 0.67 | 0.99999 |
Target: 5'- aCGCUGCU---AGAgUUUGCAUUCAAa -3' miRNA: 3'- -GUGGCGGguuUCUaAAACGUAAGUUg -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 19025 | 0.67 | 0.999988 |
Target: 5'- uCGCUGCUCAAcgaacugaccagcgcAGAUUUUGCcgcCAGCg -3' miRNA: 3'- -GUGGCGGGUU---------------UCUAAAACGuaaGUUG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 132336 | 0.68 | 0.999973 |
Target: 5'- aUACCGUCC--GGGUUU--CAUUCAACa -3' miRNA: 3'- -GUGGCGGGuuUCUAAAacGUAAGUUG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 38794 | 0.68 | 0.999951 |
Target: 5'- aACCaCCCAAGGAUaugaGCGuUUCAACa -3' miRNA: 3'- gUGGcGGGUUUCUAaaa-CGU-AAGUUG- -5' |
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16637 | 3' | -45.6 | NC_004156.1 | + | 20370 | 0.68 | 0.999951 |
Target: 5'- gCACCGCCUAAAGGa---GCucaaUCGGCa -3' miRNA: 3'- -GUGGCGGGUUUCUaaaaCGua--AGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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