Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16637 | 3' | -45.6 | NC_004156.1 | + | 223002 | 0.69 | 0.999923 |
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Target: 5'- uCACCGCucaagcgucguagauCCAAAGAUUUUGaCAUg-GACa -3' miRNA: 3'- -GUGGCG---------------GGUUUCUAAAAC-GUAagUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 90882 | 0.69 | 0.999852 |
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Target: 5'- gCACUGCCa------UUUGCAUUCAACa -3' miRNA: 3'- -GUGGCGGguuucuaAAACGUAAGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 193077 | 0.69 | 0.999809 |
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Target: 5'- cCACUGCCCGA--GUUUUGCAa--GACu -3' miRNA: 3'- -GUGGCGGGUUucUAAAACGUaagUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 145086 | 0.71 | 0.99923 |
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Target: 5'- cCAUCGCCUcuGGAUaguugGCGUUUAGCa -3' miRNA: 3'- -GUGGCGGGuuUCUAaaa--CGUAAGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 64941 | 0.73 | 0.992405 |
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Target: 5'- aUACCGCCCGAgcAGA---UGCG-UCAACu -3' miRNA: 3'- -GUGGCGGGUU--UCUaaaACGUaAGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 104914 | 0.74 | 0.989947 |
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Target: 5'- uCACCGCCauaGAGGAgccgUUGCAU-CAAUg -3' miRNA: 3'- -GUGGCGGg--UUUCUaa--AACGUAaGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 22019 | 0.75 | 0.983204 |
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Target: 5'- uCACCcaaCCAAAGAgUUUGCAUUUGACg -3' miRNA: 3'- -GUGGcg-GGUUUCUaAAACGUAAGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 67615 | 1.12 | 0.025646 |
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Target: 5'- cCACCGCCCAAAGAUUUUGCAUUCAACa -3' miRNA: 3'- -GUGGCGGGUUUCUAAAACGUAAGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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