Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16637 | 3' | -45.6 | NC_004156.1 | + | 127495 | 0.67 | 0.999995 |
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Target: 5'- aCGCUgauGCCUcagauuauAAAGAUUUUGCucUUCAACu -3' miRNA: 3'- -GUGG---CGGG--------UUUCUAAAACGu-AAGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 102177 | 0.67 | 0.999993 |
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Target: 5'- aCACUGCCUuuAGAUg--GCA--CAACa -3' miRNA: 3'- -GUGGCGGGuuUCUAaaaCGUaaGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 159485 | 0.67 | 0.99999 |
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Target: 5'- aCGCUGCU---AGAgUUUGCAUUCAAa -3' miRNA: 3'- -GUGGCGGguuUCUaAAACGUAAGUUg -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 38794 | 0.68 | 0.999951 |
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Target: 5'- aACCaCCCAAGGAUaugaGCGuUUCAACa -3' miRNA: 3'- gUGGcGGGUUUCUAaaa-CGU-AAGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 223002 | 0.69 | 0.999923 |
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Target: 5'- uCACCGCucaagcgucguagauCCAAAGAUUUUGaCAUg-GACa -3' miRNA: 3'- -GUGGCG---------------GGUUUCUAAAAC-GUAagUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 104914 | 0.74 | 0.989947 |
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Target: 5'- uCACCGCCauaGAGGAgccgUUGCAU-CAAUg -3' miRNA: 3'- -GUGGCGGg--UUUCUaa--AACGUAaGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 67615 | 1.12 | 0.025646 |
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Target: 5'- cCACCGCCCAAAGAUUUUGCAUUCAACa -3' miRNA: 3'- -GUGGCGGGUUUCUAAAACGUAAGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 75760 | 0.66 | 0.999999 |
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Target: 5'- aGCUGCgCGAguuGGAUcggUGCAUUCAGg -3' miRNA: 3'- gUGGCGgGUU---UCUAaa-ACGUAAGUUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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