Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16637 | 3' | -45.6 | NC_004156.1 | + | 169888 | 0.67 | 0.999993 |
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Target: 5'- cCACUGCCCGgcuuGAGcAUcUUGCcgUCGAg -3' miRNA: 3'- -GUGGCGGGU----UUC-UAaAACGuaAGUUg -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 175145 | 0.66 | 0.999998 |
|
Target: 5'- -gUCGUCCAGAGAg--UGCAUcuUCAuGCa -3' miRNA: 3'- guGGCGGGUUUCUaaaACGUA--AGU-UG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 180834 | 0.67 | 0.999995 |
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Target: 5'- --gCGUCCAAAGugaUUGCAUgCAACa -3' miRNA: 3'- gugGCGGGUUUCuaaAACGUAaGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 186518 | 0.66 | 0.999997 |
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Target: 5'- -uCUGCCCAuAGAUUUgaaGCAcUUUGACa -3' miRNA: 3'- guGGCGGGUuUCUAAAa--CGU-AAGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 193077 | 0.69 | 0.999809 |
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Target: 5'- cCACUGCCCGA--GUUUUGCAa--GACu -3' miRNA: 3'- -GUGGCGGGUUucUAAAACGUaagUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 207161 | 0.67 | 0.999993 |
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Target: 5'- cUACCGCCaaggguGGAGGUUUUGaauacUUCGGCa -3' miRNA: 3'- -GUGGCGGg-----UUUCUAAAACgu---AAGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 210189 | 0.66 | 0.999999 |
|
Target: 5'- gCACUGCCCGAucuggcAGAggccGCAgccgCAGCa -3' miRNA: 3'- -GUGGCGGGUU------UCUaaaaCGUaa--GUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 223002 | 0.69 | 0.999923 |
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Target: 5'- uCACCGCucaagcgucguagauCCAAAGAUUUUGaCAUg-GACa -3' miRNA: 3'- -GUGGCG---------------GGUUUCUAAAAC-GUAagUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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