Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16637 | 3' | -45.6 | NC_004156.1 | + | 31882 | 0.66 | 0.999998 |
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Target: 5'- aGCCGCCCu--GGU--UGCAgagugCAACc -3' miRNA: 3'- gUGGCGGGuuuCUAaaACGUaa---GUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 81186 | 0.66 | 0.999998 |
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Target: 5'- aCGCCGgCCAcugcagugacuaGGGAcacgUUUGgAUUCAGCg -3' miRNA: 3'- -GUGGCgGGU------------UUCUa---AAACgUAAGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 73353 | 0.66 | 0.999999 |
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Target: 5'- aGCCGUCCucAGuAUgguacucGCGUUCGACa -3' miRNA: 3'- gUGGCGGGuuUC-UAaaa----CGUAAGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 165882 | 0.66 | 0.999999 |
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Target: 5'- gACgCGCUCGAAGugUUUGguUUCGAUg -3' miRNA: 3'- gUG-GCGGGUUUCuaAAACguAAGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 18720 | 0.66 | 0.999999 |
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Target: 5'- cUACCGCCCAuAGAUg-UGaCcgUgAGCg -3' miRNA: 3'- -GUGGCGGGUuUCUAaaAC-GuaAgUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 136155 | 0.66 | 0.999999 |
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Target: 5'- gGCCGCCgAGGGAaugggauagagUGCAUugUCGAUa -3' miRNA: 3'- gUGGCGGgUUUCUaaa--------ACGUA--AGUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 210189 | 0.66 | 0.999999 |
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Target: 5'- gCACUGCCCGAucuggcAGAggccGCAgccgCAGCa -3' miRNA: 3'- -GUGGCGGGUU------UCUaaaaCGUaa--GUUG- -5' |
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| 16637 | 3' | -45.6 | NC_004156.1 | + | 75760 | 0.66 | 0.999999 |
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Target: 5'- aGCUGCgCGAguuGGAUcggUGCAUUCAGg -3' miRNA: 3'- gUGGCGgGUU---UCUAaa-ACGUAAGUUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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