Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16647 | 3' | -52 | NC_004156.1 | + | 163593 | 0.66 | 0.993297 |
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Target: 5'- gGCUCAGUuuuACUCGUACG---AugGCAa -3' miRNA: 3'- aCGGGUCA---UGAGCAUGUgguUugCGU- -5' |
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| 16647 | 3' | -52 | NC_004156.1 | + | 40614 | 0.66 | 0.993297 |
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Target: 5'- cGUCCgAGUAgaUCGUcuACACCAAACGa- -3' miRNA: 3'- aCGGG-UCAUg-AGCA--UGUGGUUUGCgu -5' |
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| 16647 | 3' | -52 | NC_004156.1 | + | 158390 | 0.66 | 0.993297 |
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Target: 5'- aGUCCAGUguauagACguugUGUGUACCGAAUGCAa -3' miRNA: 3'- aCGGGUCA------UGa---GCAUGUGGUUUGCGU- -5' |
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| 16647 | 3' | -52 | NC_004156.1 | + | 95069 | 0.66 | 0.993297 |
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Target: 5'- aGCCCAGUAC-CGUugcagaggcCAUCAAACuGUAc -3' miRNA: 3'- aCGGGUCAUGaGCAu--------GUGGUUUG-CGU- -5' |
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| 16647 | 3' | -52 | NC_004156.1 | + | 11305 | 0.66 | 0.993297 |
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Target: 5'- cUGCaCCGGagaggGUGCAUCAAACGCAu -3' miRNA: 3'- -ACG-GGUCaugagCAUGUGGUUUGCGU- -5' |
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| 16647 | 3' | -52 | NC_004156.1 | + | 44957 | 0.66 | 0.994186 |
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Target: 5'- cGCCUGGUACg---GCACCGGuCGCc -3' miRNA: 3'- aCGGGUCAUGagcaUGUGGUUuGCGu -5' |
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| 16647 | 3' | -52 | NC_004156.1 | + | 198225 | 0.66 | 0.994977 |
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Target: 5'- gUGUCCAGU-CUCGUugG-CAGAgGCu -3' miRNA: 3'- -ACGGGUCAuGAGCAugUgGUUUgCGu -5' |
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| 16647 | 3' | -52 | NC_004156.1 | + | 216708 | 0.66 | 0.995678 |
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Target: 5'- -cUUCGGUGCUCGUAC-CCAAACc-- -3' miRNA: 3'- acGGGUCAUGAGCAUGuGGUUUGcgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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