Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16647 | 3' | -52 | NC_004156.1 | + | 174520 | 0.67 | 0.987106 |
Target: 5'- uUGCCCucGUACUCGUcGCGuuggugguCCA-GCGCAu -3' miRNA: 3'- -ACGGGu-CAUGAGCA-UGU--------GGUuUGCGU- -5' |
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16647 | 3' | -52 | NC_004156.1 | + | 175763 | 0.67 | 0.987106 |
Target: 5'- cGUCUagacGGUGCUCGUcCACCGAACu-- -3' miRNA: 3'- aCGGG----UCAUGAGCAuGUGGUUUGcgu -5' |
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16647 | 3' | -52 | NC_004156.1 | + | 190724 | 0.8 | 0.495747 |
Target: 5'- uUGCCgAGUccccggACUCGUcuACACCGAACGCGu -3' miRNA: 3'- -ACGGgUCA------UGAGCA--UGUGGUUUGCGU- -5' |
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16647 | 3' | -52 | NC_004156.1 | + | 198225 | 0.66 | 0.994977 |
Target: 5'- gUGUCCAGU-CUCGUugG-CAGAgGCu -3' miRNA: 3'- -ACGGGUCAuGAGCAugUgGUUUgCGu -5' |
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16647 | 3' | -52 | NC_004156.1 | + | 211567 | 0.67 | 0.987106 |
Target: 5'- gUGCUCuaucgucgucGUugUCGUACuguuCCAGAUGCAu -3' miRNA: 3'- -ACGGGu---------CAugAGCAUGu---GGUUUGCGU- -5' |
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16647 | 3' | -52 | NC_004156.1 | + | 213895 | 0.68 | 0.983466 |
Target: 5'- cGCCagAGUACUggugcguacCGUGCACCGAGCcgagacgGCAa -3' miRNA: 3'- aCGGg-UCAUGA---------GCAUGUGGUUUG-------CGU- -5' |
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16647 | 3' | -52 | NC_004156.1 | + | 214139 | 0.68 | 0.977227 |
Target: 5'- cGCaCCAGUACUCugGCGUCGAACGUu -3' miRNA: 3'- aCG-GGUCAUGAGcaUGUGGUUUGCGu -5' |
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16647 | 3' | -52 | NC_004156.1 | + | 216708 | 0.66 | 0.995678 |
Target: 5'- -cUUCGGUGCUCGUAC-CCAAACc-- -3' miRNA: 3'- acGGGUCAUGAGCAUGuGGUUUGcgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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