Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16656 | 3' | -57.1 | NC_004156.1 | + | 110253 | 0.68 | 0.842411 |
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Target: 5'- cUGCuGGGCCACaGACACaaCGGAGCcCg -3' miRNA: 3'- cACGuUCUGGUGgCUGUG--GCCUCGcG- -5' |
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| 16656 | 3' | -57.1 | NC_004156.1 | + | 129949 | 0.69 | 0.834502 |
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Target: 5'- aUGCAAGACUAcaaugugcuacCCGuacCGCCGGGGaGCa -3' miRNA: 3'- cACGUUCUGGU-----------GGCu--GUGGCCUCgCG- -5' |
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| 16656 | 3' | -57.1 | NC_004156.1 | + | 134131 | 0.69 | 0.834502 |
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Target: 5'- -cGCAAGugCACCGACACCauacucuUGCa -3' miRNA: 3'- caCGUUCugGUGGCUGUGGccuc---GCG- -5' |
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| 16656 | 3' | -57.1 | NC_004156.1 | + | 13959 | 0.69 | 0.826423 |
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Target: 5'- uGUGCu-GACCACCaaGCACCGaGGCGa -3' miRNA: 3'- -CACGuuCUGGUGGc-UGUGGCcUCGCg -5' |
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| 16656 | 3' | -57.1 | NC_004156.1 | + | 157452 | 0.7 | 0.747195 |
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Target: 5'- cUGCAAGACUAuUUGGCAUCGGAGaGUa -3' miRNA: 3'- cACGUUCUGGU-GGCUGUGGCCUCgCG- -5' |
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| 16656 | 3' | -57.1 | NC_004156.1 | + | 206053 | 0.7 | 0.737813 |
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Target: 5'- cUGCAucggAGAgUACCGGCACUGGuaccGGUGCu -3' miRNA: 3'- cACGU----UCUgGUGGCUGUGGCC----UCGCG- -5' |
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| 16656 | 3' | -57.1 | NC_004156.1 | + | 127046 | 0.7 | 0.737813 |
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Target: 5'- -cGCGAGcAUCACCGGCAaacacuagCGGAGaCGCu -3' miRNA: 3'- caCGUUC-UGGUGGCUGUg-------GCCUC-GCG- -5' |
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| 16656 | 3' | -57.1 | NC_004156.1 | + | 129064 | 0.72 | 0.6702 |
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Target: 5'- cGUGUAaaacAGAUUAcCCGACACCaGAGUGCc -3' miRNA: 3'- -CACGU----UCUGGU-GGCUGUGGcCUCGCG- -5' |
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| 16656 | 3' | -57.1 | NC_004156.1 | + | 106884 | 0.73 | 0.601147 |
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Target: 5'- -cGCAGGAgCACCGGCA---GAGCGCa -3' miRNA: 3'- caCGUUCUgGUGGCUGUggcCUCGCG- -5' |
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| 16656 | 3' | -57.1 | NC_004156.1 | + | 201961 | 1.11 | 0.002562 |
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Target: 5'- cGUGCAAGACCACCGACACCGGAGCGCu -3' miRNA: 3'- -CACGUUCUGGUGGCUGUGGCCUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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