Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16700 | 3' | -54.5 | NC_004156.1 | + | 199627 | 0.71 | 0.824109 |
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Target: 5'- uCGGcAUCACGCAGAC--GGACGAgGACc -3' miRNA: 3'- -GUC-UGGUGCGUCUGagUCUGCUgCUG- -5' |
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| 16700 | 3' | -54.5 | NC_004156.1 | + | 7566 | 0.71 | 0.824109 |
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Target: 5'- aCAGACCAauacUGUAGAaccauuaCAGACGGCGAUg -3' miRNA: 3'- -GUCUGGU----GCGUCUga-----GUCUGCUGCUG- -5' |
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| 16700 | 3' | -54.5 | NC_004156.1 | + | 122915 | 0.72 | 0.771259 |
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Target: 5'- uCAGAUgGCGguGuCUCGGACGAUGcCg -3' miRNA: 3'- -GUCUGgUGCguCuGAGUCUGCUGCuG- -5' |
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| 16700 | 3' | -54.5 | NC_004156.1 | + | 150502 | 0.73 | 0.752596 |
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Target: 5'- aGGGCUACGCAGugUUGGACGuaaaggaggGCGAa -3' miRNA: 3'- gUCUGGUGCGUCugAGUCUGC---------UGCUg -5' |
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| 16700 | 3' | -54.5 | NC_004156.1 | + | 172816 | 0.73 | 0.743106 |
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Target: 5'- aAGACgAUGgAGACUaugaAGACGAUGACg -3' miRNA: 3'- gUCUGgUGCgUCUGAg---UCUGCUGCUG- -5' |
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| 16700 | 3' | -54.5 | NC_004156.1 | + | 211179 | 1.11 | 0.005013 |
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Target: 5'- aCAGACCACGCAGACUCAGACGACGACg -3' miRNA: 3'- -GUCUGGUGCGUCUGAGUCUGCUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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